ArticleGenetics1998
Genetic analysis of chloroplast c-type cytochrome assembly in Chlamydomonas reinhardtii: One chloroplast locus and at least four nuclear loci are required for heme attachment.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- Analysis of putative heme ligands in the System I bacterial cytochromeMicrobiology spectrum · 2026Article
- The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025Review
- Helicobacter pylori and Campylobacter jejuni bacterial holocytochrome c synthase structure-function analysis reveals conservation of heme binding.Communications biology · 2024Article
- Structural basis of membrane machines that traffick and attach heme to cytochromes.The Journal of biological chemistry · 2023Article
- Two disulfide-reducing pathways are required for the maturation of plastid c-type cytochromes in Chlamydomonas reinhardtii.Genetics · 2023Article
- Cryo-EM of CcsBA reveals the basis for cytochrome c biogenesis and heme transport.Nature chemical biology · 2022Article
- Article
- Phylogenomic Analysis and Dynamic Evolution of Chloroplast Genomes in Salicaceae.Frontiers in plant science · 2017Article
- CCS2, an Octatricopeptide-Repeat Protein, Is Required for Plastid CytochromeFrontiers in plant science · 2017Article
- Maturation of PlastidFrontiers in plant science · 2017Review
- High-Resolution Profiling of a Synchronized Diurnal Transcriptome from Chlamydomonas reinhardtii Reveals Continuous Cell and Metabolic Differentiation.The Plant cell · 2015Article
- Nitric oxide-triggered remodeling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas reinhardtii.The Plant cell · 2014Article
- Operation of trans-thylakoid thiol-metabolizing pathways in photosynthesis.Frontiers in plant science · 2013Article
- Regulatory factors for the assembly of thylakoid membrane protein complexes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- A novel component of the disulfide-reducing pathway required for cytochrome c assembly in plastids.Genetics · 2011Article
- The nucleus-encoded trans-acting factor MCA1 plays a critical role in the regulation of cytochrome f synthesis in Chlamydomonas chloroplasts.The Plant cell · 2011Article
- Tetrapyrrole Metabolism in Arabidopsis thaliana.The arabidopsis book · 2011Article
- CCS5, a thioredoxin-like protein involved in the assembly of plastid c-type cytochromes.The Journal of biological chemistry · 2010Article
- Cytochrome c biogenesis: mechanisms for covalent modifications and trafficking of heme and for heme-iron redox control.Microbiology and molecular biology reviews : MMBR · 2009Review
- CcsBA is a cytochrome c synthetase that also functions in heme transport.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Chloroplasts contain up to two c-type cytochromes, membrane-anchored cytochrome f and soluble cytochrome c6. To elucidate the post-translational events required for their assembly, acetate-requiring mutants of Chlamydomonas reinhardtii that have combined deficiencies in both plastid-encoded cytochrome f and nucleus-encoded cytochrome c6 have been identified and analyzed. For strains ct34 and ct59, where the phenotype displays uniparental inheritance, the mutations were localized to the chloroplast ccsA gene, which was shown previously to be required for heme attachment to chloroplast apocytochromes. The mutations in another eight strains were localized to the nuclear genome. Complementation tests of these strains plus three previously identified strains of the same phenotype (ac206, F18, and F2D8) indicate that the 11 ccs strains define four nuclear loci, CCS1-CCS4. We conclude that the products of the CCS1-CCS4 loci are not required for translocation or processing of the preproteins but, like CcsA, they are required for the heme attachment step during assembly of both holocytochrome f and holocytochrome c6. The ccsA gene is transcribed in each of the nuclear mutants, but its protein product is absent in ccs1 mutants, and it appears to be degradation susceptible in ccs3 and ccs4 strains. We suggest that Ccsl may be associated with CcsA in a multisubunit "holocytochrome c assembly complex," and we hypothesize that the products of the other CCS loci may correspond to other subunits.
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