ArticleThe Journal of physiology1998
NH4+ as a substrate for apical and basolateral Na(+)-H+ exchangers of thick ascending limbs of rat kidney: evidence from isolated membranes.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- The SLC-ome of membrane transport: from molecular discovery to physiology and clinical applications.Physiological reviews · 2026Review
- Mechanisms and physiological relevance of acid-base exchange in functional units of the kidney.PeerJ · 2024Review
- The Role of Plasma Membrane Sodium/Hydrogen Exchangers in Gastrointestinal Functions: Proliferation and Differentiation, Fluid/Electrolyte Transport and Barrier Integrity.Frontiers in physiology · 2022Review
- Effects of ocean acidification and salinity variations on the physiology of osmoregulating and osmoconforming crustaceans.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- The importance of the thick ascending limb of Henle's loop in renal physiology and pathophysiology.International journal of nephrology and renovascular disease · 2018Review
- Ammonia Transporters and Their Role in Acid-Base Balance.Physiological reviews · 2017Review
- Renal acid-base regulation: new insights from animal models.Pflugers Archiv : European journal of physiology · 2015Review
- Everything you need to know about distal renal tubular acidosis in autoimmune disease.Rheumatology international · 2014Review
- Interactions of ion transporters and channels with cancer cell metabolism and the tumour microenvironment.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Proximal tubule specific knockout of the Na⁺/H⁺ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion.Journal of molecular medicine (Berlin, Germany) · 2013Article
- Role of NH3 and NH4+ transporters in renal acid-base transport.American journal of physiology. Renal physiology · 2011Review
- NHE4 is critical for the renal handling of ammonia in rodents.The Journal of clinical investigation · 2010Article
- A mathematical model of rat ascending Henle limb. II. Epithelial function.American journal of physiology. Renal physiology · 2010Article
- Expression of the human erythroid Rh glycoprotein (RhAG) enhances both NH3 and NH4+ transport in HeLa cells.Pflugers Archiv : European journal of physiology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. We have used highly purified right-side-out luminal and basolateral membrane vesicles (LMVs and BLMVs) isolated from rat medullary thick ascending limb (MTAL) to study directly the possible roles of the LMV and BLMV Na(+)-H+ exchangers in the transport of NH4+. 2. Extravesicular NH4+ ((NH4+)o) inhibited outward H+ gradient-stimulated 22Na+ uptake in both types of vesicles. This inhibition could not be accounted for by alteration of intravesicular pH (pHi). 3. Conversely, in both plasma membrane preparations, the imposition of outward NH4+ gradients stimulated 22Na+ uptake at the acidic pHi (6.60) of MTAL cells, under conditions in which possible alterations in pHi were prevented. All NH4+ gradient-stimulated Na+ uptake was sensitive to 0.5 mM 5-(N,N-dimethyl)-amiloride. 4. The BLMV and LMV Na(+)-H+ exchangers had a similar apparent affinity for internal H+ (Hi+), with pK (-log of dissociation constant) values of 6.58 and 6.52, respectively. 5. These findings indicate that NH4+ interacts with the external and internal transport sites of the LMV and BLMV Na(+)-H+ antiporters, and that both of these exchangers can mediate the exchange of internal NH4+ ((NH4+)i) for external Na+ (Na+o) at the prevailing pHi of MTAL cells. 6. We conclude that operation of the BLMV Na(+)-H+ exchanger on the NH4(+)-Na+ mode may represent an important pathway for mediating the final step of NH4+ absorption, whereas transport of NH4+ on the apical antiporter may provide negative feedback regulation of NH4+ absorption.
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