ArticleMammalian genome : official journal of the International Mammalian Genome Society1998
Utility of C57BL/6J x 129/SvJae embryonic stem cells for generating chromosomal deletions: tolerance to gamma radiation and microsatellite polymorphism.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
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- Esrrb-Cre excises loxP-flanked alleles in early four-cell embryos.Genesis (New York, N.Y. : 2000) · 2016Article
- Adipocyte-specific disruption of fat-specific protein 27 causes hepatosteatosis and insulin resistance in high-fat diet-fed mice.The Journal of biological chemistry · 2015Article
- Secretoglobin superfamily protein SCGB3A2 deficiency potentiates ovalbumin-induced allergic pulmonary inflammation.Mediators of inflammation · 2014Article
- Mixture of differentially tagged Tol2 transposons accelerates conditional disruption of a broad spectrum of genes in mouse embryonic stem cells.Nucleic acids research · 2012Article
- Risks and mechanisms of oncological disease following stem cell transplantation.Stem cell reviews and reports · 2010Review
- Gene targeting in mouse embryonic stem cells.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Modeling chromosomes in mouse to explore the function of genes, genomic disorders, and chromosomal organization.PLoS genetics · 2006Review
- The use of unilateral PCR to identify prominent heteroduplexes formed during PCR of the mouse microsatellite locus D17Mit23.Molecular biotechnology · 2006Article
- X-ray-induced deletion complexes in embryonic stem cells on mouse chromosome 15.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
- Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.Nucleic acids research · 2005Article
- Overlapping deletions spanning the proximal two-thirds of the mouse t complex.Mammalian genome : official journal of the International Mammalian Genome Society · 2003Article
- Article
- Interdigitated deletion complexes on mouse chromosome 5 induced by irradiation of embryonic stem cells.Genome research · 2000Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have previously reported a method for making nested deletion complexes in mice by irradiation of ES cells. The key to this technology is that F1 hybrid ES cells (called v17.2) of the genotype (BALB/cTa x 129/SvJae) retain germline colonizing ability after exposure to levels of ionizing radiation that induce chromosomal deletions. In an effort to identify other genotypes of ES cells that are suitable for this technology, the radiation sensitivity of the cell line v6.4, which is of the genotype (C57BL/6J x 129/SvJae), was investigated. After treatment with a range of radiation exposures, the developmental potential of these cells was assayed by injecting them into blastocysts to generate chimeric mice. These experiments showed that while cell lethality increased as the level of radiation increased, the surviving ES cells retained full totipotency at all exposure levels, up to 400 Rads. Because polymorphism between parental microsatellite alleles in the F1 hybrid ES cells is important for ascertaining the sizes of induced deletions, the 129/SvJ and 129/SvJae allele sizes of 48 microsatellite loci on chromosome (Chr) 17 were determined. This revealed a higher level of polymorphism between 129 and C57BL/6J on Chr 17. The radiation tolerance, high polymorphism between parental strains, and presence of the widely used C57BL/6J strain component make v6.4 ES cells an attractive cell line for generating radiation-induced chromosomal deletions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.