ArticleProceedings of the National Academy of Sciences of the United States of America1998
Subcellular localization of mineralocorticoid receptors in living cells: effects of receptor agonists and antagonists.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 247 citations in OpenAlex.
- Mineralocorticoid Receptor Activation Contributes to the Supine Hypertension of Autonomic Failure.Hypertension (Dallas, Tex. : 1979) · 2016Trial
- Endocrine Examples of Phase Separation in Biology.Endocrinology · 2025Review
- Control of sodium appetite by hindbrain aldosterone-sensitive neurons.Molecular and cellular endocrinology · 2024Review
- The mineralocorticoid receptor forms higher order oligomers upon DNA binding.Protein science : a publication of the Protein Society · 2024Article
- MicroRNA-19 is regulated by aldosterone in a sex-specific manner to alter kidney sodium transport.American journal of physiology. Cell physiology · 2024Article
- Protein Condensation in the Nuclear Receptor Family; Implications for Transcriptional Output.Advances in experimental medicine and biology · 2022Article
- Choosing the right partner in hormone-dependent gene regulation: Glucocorticoid and progesterone receptors crosstalk in breast cancer cells.Frontiers in endocrinology · 2022Review
- Non-coding RNAs and the mineralocorticoid receptor in the kidney.Molecular and cellular endocrinology · 2021Review
- Posttranslational Modifications of the Mineralocorticoid Receptor and Cardiovascular Aging.Frontiers in molecular biosciences · 2021Review
- Aldosterone-induced microRNAs act as feedback regulators of mineralocorticoid receptor signaling in kidney epithelia.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Inhibitors of Brassinosteroid Biosynthesis and Signal Transduction.Molecules (Basel, Switzerland) · 2019Review
- Aldosterone and Mineralocorticoid Receptor System in Cardiovascular Physiology and Pathophysiology.Oxidative medicine and cellular longevity · 2018Review
- HuR-Dependent Editing of a New Mineralocorticoid Receptor Splice Variant Reveals an Osmoregulatory Loop for Sodium Homeostasis.Scientific reports · 2017Article
- The Pharmacology of Autonomic Failure: From Hypotension to Hypertension.Pharmacological reviews · 2017Review
- Finerenone Impedes Aldosterone-dependent Nuclear Import of the Mineralocorticoid Receptor and Prevents Genomic Recruitment of Steroid Receptor Coactivator-1.The Journal of biological chemistry · 2015Article
- The multifaceted mineralocorticoid receptor.Comprehensive Physiology · 2014Review
- Behavioral stress induces regionally-distinct shifts of brain mineralocorticoid and glucocorticoid receptor levels.Frontiers in behavioral neuroscience · 2014Article
- A new strategy for selective targeting of progesterone receptor with passive antagonists.Molecular endocrinology (Baltimore, Md.) · 2013Article
- The diuretic torasemide does not prevent aldosterone-mediated mineralocorticoid receptor activation in cardiomyocytes.PloS one · 2013Article
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Results on the subcellular localization of the mineralocorticoid receptor (MR) have been controversial. To determine the subcellular distribution and trafficking of the MR in living cells after binding of agonists and antagonists, we expressed a MR-green fluorescent protein (GFP) chimera in mammalian cells lacking endogenous MR. The GFP-tagged MR (GFP-MR) remained transcriptionally active, as determined in cotransfection experiments with the MR-responsive reporter, TAT3-LUC. The subcellular localization of GFP-MR was monitored by fluorescence time-lapse microscopy. In the absence of hormone, MR was present both in the cytoplasm and nucleus. Aldosterone induced a rapid nuclear accumulation of the MR. Aldosterone-bound GFP-MR was concentrated in prominent clusters within the nucleus, whereas GFP-MR did not form clusters in the absence of hormone. Similar subnuclear distribution was observed with corticosterone, another MR agonist. In the presence of the MR antagonists spironolactone or ZK91587 the rate of nuclear translocation was significantly slower and the final nuclear-to-cytoplasmic ratio in steady state was significantly lower than with aldosterone. In addition, MR antagonists did not induce formation of nuclear GFP-MR clusters. MR antagonists also were able to disrupt pre-existing nuclear clusters formed in the presence of aldosterone. GFP-MR clusters were retained in nuclear matrix preparations after in vivo crosslinking. These data strongly suggest that hormone-activated MRs accumulate in dynamic discrete clusters in the cell nucleus, and this phenomenon occurs only with transcriptionally active mineralocorticoids.
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