ArticlePlant physiology1998
Biochemical characterization of stromal and thylakoid-bound isoforms of isoprene synthase in willow leaves
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 78 citations in OpenAlex.
- Isoprene: New insights into the control of emission and mediation of stress tolerance by gene expression.Plant, cell & environment · 2019Review
- Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha.BMC plant biology · 2018Article
- Increased thermostability of thylakoid membranes in isoprene-emitting leaves probed with three biophysical techniques.Plant physiology · 2011Article
- Temperature response of isoprene emission in vivo reflects a combined effect of substrate limitations and isoprene synthase activity: a kinetic analysis.Plant physiology · 2010Article
- Genetic structure and regulation of isoprene synthase in Poplar (Populus spp.).Plant molecular biology · 2010Article
- Evidence that light, carbon dioxide, and oxygen dependencies of leaf isoprene emission are driven by energy status in hybrid aspen.Plant physiology · 2009Article
- Postillumination isoprene emission: in vivo measurements of dimethylallyldiphosphate pool size and isoprene synthase kinetics in aspen leaves.Plant physiology · 2009Article
- Biosynthesis, accumulation and emission of carotenoids, alpha-tocopherol, plastoquinone, and isoprene in leaves under high photosynthetic irradiance.Photosynthesis research · 2007Review
- Article
- Evolution of the isoprene biosynthetic pathway in kudzu.Plant physiology · 2005Article
- Rapid regulation of the methylerythritol 4-phosphate pathway during isoprene synthesis.Plant physiology · 2004Article
- Ozone quenching properties of isoprene and its antioxidant role in leaves.Plant physiology · 2001Article
- Thermotolerance of leaf discs from four isoprene-emitting species is not enhanced by exposure to exogenous isoprenePlant physiology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoprene synthase is the enzyme responsible for the foliar emission of the hydrocarbon isoprene (2-methyl-1,3-butadiene) from many C3 plants. Previously, thylakoid-bound and soluble forms of isoprene synthase had been isolated separately, each from different plant species using different procedures. Here we describe the isolation of thylakoid-bound and soluble isoprene synthases from a single willow (Salix discolor L.) leaf-fractionation protocol. Willow leaf isoprene synthase appears to be plastidic, with whole-leaf and intact chloroplast fractionations yielding approximately equal soluble (i.e. stromal) and thylakoid-bound isoprene synthase activities. Although thylakoid-bound isoprene synthase is tightly bound to the thylakoid membrane (M.C. Wildermuth, R. Fall [1996] Plant Physiol 112: 171-182), it can be solubilized by pH 10.0 treatment. The solubilized thylakoid-bound and stromal isoprene synthases exhibit similar catalytic properties, and contain essential cysteine, histidine, and arginine residues, as do other isoprenoid synthases. In addition, two regulators of foliar isoprene emission, leaf age and light, do not alter the percentage of isoprene synthase activity in the bound or soluble form. The relationship between the isoprene synthase isoforms and the implications for function and regulation of isoprene production are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.