ArticlePlant physiology1998
An embryo-defective mutant of arabidopsis disrupted in the final step of biotin synthesis
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 97 citations in OpenAlex.
- Best practices for biotin ligase-based proximity labeling proteomics in plant systems.The Plant cell · 2026Review
- A brief guide to analyzing TurboID-based proximity labeling-mass spectrometry in plants.Molecules and cells · 2025Review
- The function of glutaredoxin GRXS15 is required for lipoyl-dependent dehydrogenases in mitochondria.Plant physiology · 2021Article
- Plastidial NAD-Dependent Malate Dehydrogenase: A Moonlighting Protein Involved in Early Chloroplast Development through Its Interaction with an FtsH12-FtsHi Protease Complex.The Plant cell · 2018Article
- Article
- Natural variation in sensitivity to a loss of chloroplast translation in Arabidopsis.Plant physiology · 2014Article
- SUCROSE TRANSPORTER 5 supplies Arabidopsis embryos with biotin and affects triacylglycerol accumulation.The Plant journal : for cell and molecular biology · 2013Article
- Expanding functional repertoires of fungal peroxisomes: contribution to growth and survival processes.Frontiers in physiology · 2013Article
- A newly discovered function of peroxisomes: involvement in biotin biosynthesis.Plant signaling & behavior · 2012Review
- Article
- Role of cis-12-oxo-phytodienoic acid in tomato embryo development.Plant physiology · 2012Article
- Reverse-genetic analysis of the two biotin-containing subunit genes of the heteromeric acetyl-coenzyme A carboxylase in Arabidopsis indicates a unidirectional functional redundancy.Plant physiology · 2011Article
- System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolism.Plant physiology · 2009Article
- Proteome-wide characterization of seed aging in Arabidopsis: a comparison between artificial and natural aging protocols.Plant physiology · 2008Article
- A bifunctional locus (BIO3-BIO1) required for biotin biosynthesis in Arabidopsis.Plant physiology · 2008Article
- Genetic dissection of histidine biosynthesis in Arabidopsis.Plant physiology · 2007Article
- Functional analysis of PDX2 from Arabidopsis, a glutaminase involved in vitamin B6 biosynthesis.Plant physiology · 2007Article
- The role of plant mitochondria in the biosynthesis of coenzymes.Photosynthesis research · 2007Review
- The Arabidopsis Bio2 protein requires mitochondrial targeting for activity.Plant molecular biology · 2006Article
- Biotin synthesis in plants. The first committed step of the pathway is catalyzed by a cytosolic 7-keto-8-aminopelargonic acid synthase.Plant physiology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Auxotrophic mutants have played an important role in the genetic dissection of biosynthetic pathways in microorganisms. Equivalent mutants have been more difficult to identify in plants. The bio1 auxotroph of Arabidopsis thaliana was shown previously to be defective in the synthesis of the biotin precursor 7, 8-diaminopelargonic acid. A second biotin auxotroph of A. thaliana has now been identified. Arrested embryos from this bio2 mutant are defective in the final step of biotin synthesis, the conversion of dethiobiotin to biotin. This enzymatic reaction, catalyzed by the bioB product (biotin synthase) in Escherichia coli, has been studied extensively in plants and bacteria because it involves the unusual addition of sulfur to form a thiophene ring. Three lines of evidence indicate that bio2 is defective in biotin synthase production: mutant embryos are rescued by biotin but not dethiobiotin, the mutant allele maps to the same chromosomal location as the cloned biotin synthase gene, and gel-blot hybridizations and polymerase chain reaction amplifications revealed that homozygous mutant plants contain a deletion spanning the entire BIO2-coding region. Here we describe how the isolation and characterization of this null allele have provided valuable insights into biotin synthesis, auxotrophy, and gene redundancy in plants.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.