ArticlePlant physiology1998
Characterization of SKT1, an inwardly rectifying potassium channel from potato, by heterologous expression in insect cells.
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 84 citations in OpenAlex.
- Transcriptome analysis and identification of the low potassium stress-responsive gene SiSnRK2.6 in foxtail millet (Setaria italica L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Recent Advances in Genome-wide Analyses of Plant Potassium Transporter Families.Current genomics · 2021Review
- Potassium Control of Plant Functions: Ecological and Agricultural Implications.Plants (Basel, Switzerland) · 2021Article
- Review
- NPKS uptake, sensing, and signaling and miRNAs in plant nutrient stress.Protoplasma · 2016Review
- The Os-AKT1 channel is critical for K+ uptake in rice roots and is modulated by the rice CBL1-CIPK23 complex.The Plant cell · 2014Article
- OsKAT2 is the prevailing functional inward rectifier potassium channels in rice guard cell.Plant signaling & behavior · 2013Article
- Unique features of two potassium channels, OsKAT2 and OsKAT3, expressed in rice guard cells.PloS one · 2013Article
- Involvement of the putative Ca²⁺-permeable mechanosensitive channels, NtMCA1 and NtMCA2, in Ca²⁺ uptake, Ca²⁺-dependent cell proliferation and mechanical stress-induced gene expression in tobacco (Nicotiana tabacum) BY-2 cells.Journal of plant research · 2012Article
- Roles of a putative mechanosensitive plasma membrane Ca2+-permeable channel OsMCA1 in generation of reactive oxygen species and hypo-osmotic signaling in rice.Plant signaling & behavior · 2012Article
- Expression of the AKT1-type K(+) channel gene from Puccinellia tenuiflora, PutAKT1, enhances salt tolerance in Arabidopsis.Plant cell reports · 2010Article
- Heterelogous expression of plant genes.International journal of plant genomics · 2009Article
- Stoichiometry studies reveal functional properties of KDC1 in plant shaker potassium channels.Biophysical journal · 2006Article
- Properties of shaker-type potassium channels in higher plants.The Journal of membrane biology · 2006Review
- Article
- AtKC1, a silent Arabidopsis potassium channel alpha -subunit modulates root hair K+ influx.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Internal aluminum block of plant inward K(+) channels.The Plant cell · 2001Article
- Enhancement of Na(+) uptake currents, time-dependent inward-rectifying K(+) channel currents, and K(+) channel transcripts by K(+) starvation in wheat root cells.Plant physiology · 2000Article
- The integral membrane S-locus receptor kinase of Brassica has serine/threonine kinase activity in a membranous environment and spontaneously forms oligomers in planta.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Histidine(118) in the S2-S3 linker specifically controls activation of the KAT1 channel expressed in Xenopus oocytes.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding a novel, inwardly rectifying K+ (K+in) channel protein, SKT1, was cloned from potato (Solanum tuberosum L.). SKT1 is related to members of the AKT family of K+in channels previously identified in Arabidopsis thaliana and potato. Skt1 mRNA is most strongly expressed in leaf epidermal fragments and in roots. In electrophysiological, whole-cell, patch-clamp measurements performed on baculovirus-infected insect (Spodoptera frugiperda) cells, SKT1 was identified as a K+in channel that activates with slow kinetics by hyperpolarizing voltage pulses to more negative potentials than -60 mV. The pharmacological inhibitor Cs+, when applied externally, inhibited SKT1-mediated K+in currents half-maximally with an inhibitor concentration (IC50) of 105 microM. An almost identical high Cs+ sensitivity (IC50 = 90 microM) was found for the potato guard-cell K+in channel KST1 after expression in insect cells. SKT1 currents were reversibly activated by a shift in external pH from 6.6 to 5.5, which indicates a physiological role for pH-dependent regulation of AKT-type K+in channels. Comparative studies revealed generally higher current amplitudes for KST1-expressing cells than for SKT1-expressing insect cells, which correlated with a higher targeting efficiency of the KST1 protein to the insect cell's plasma membrane, as demonstrated by fusions to green fluorescence protein.
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