ArticleJournal of virology1998
Mapping viral DNA specificity to the central region of integrase by using functional human immunodeficiency virus type 1/visna virus chimeric proteins.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- DNA strand breaks and gaps target retroviral intasome binding and integration.Nature communications · 2023Article
- Review
- Review
- Integration specificity of LTR-retrotransposons and retroviruses in the Drosophila melanogaster genome.Virus genes · 2011Article
- Mutations associated with failure of raltegravir treatment affect integrase sensitivity to the inhibitor in vitro.Antimicrobial agents and chemotherapy · 2008Article
- Small-angle X-ray characterization of the nucleoprotein complexes resulting from DNA-induced oligomerization of HIV-1 integrase.Nucleic acids research · 2007Article
- Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51.Nucleic acids research · 2006Article
- Division of labor within human immunodeficiency virus integrase complexes: determinants of catalysis and target DNA capture.Journal of virology · 2005Article
- Symmetrical recognition of cellular DNA target sequences during retroviral integration.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Evaluation of the functional involvement of human immunodeficiency virus type 1 integrase in nuclear import of viral cDNA during acute infection.Journal of virology · 2004Article
- Cross-packaging of human immunodeficiency virus type 1 vector RNA by spleen necrosis virus proteins: construction of a new generation of spleen necrosis virus-derived retroviral vectors.Journal of virology · 2004Article
- Biochemical and random mutagenesis analysis of the region carrying the catalytic E152 amino acid of HIV-1 integrase.Nucleic acids research · 2004Article
- An amino acid in the central catalytic domain of three retroviral integrases that affects target site selection in nonviral DNA.Journal of virology · 2003Article
- Assembly and catalysis of concerted two-end integration events by Moloney murine leukemia virus integrase.Journal of virology · 2001Article
- Use of patient-derived human immunodeficiency virus type 1 integrases to identify a protein residue that affects target site selection.Journal of virology · 2001Article
- Retroviral DNA integration.Microbiology and molecular biology reviews : MMBR · 1999Review
- Dissecting the role of the N-terminal domain of human immunodeficiency virus integrase by trans-complementation analysis.Journal of virology · 1999Article
- Sequence specificity of viral end DNA binding by HIV-1 integrase reveals critical regions for protein-DNA interaction.The EMBO journal · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We previously described the construction and analysis of the first set of functional chimeric lentivirus integrases, involving exchange of the N-terminal, central, and C-terminal regions of the human immunodeficiency virus type 1 (HIV-1) and visna virus integrase (IN) proteins. Based on those results, additional HIV-1/visna virus chimeric integrases were designed and purified. Each of the chimeric enzymes was functional in at least one oligonucleotide-based IN assay. Of a total of 12 chimeric IN proteins, 3 exhibit specific viral DNA processing, 9 catalyze insertion of viral DNA ends, 12 can reverse that reaction, and 11 are active for nonspecific alcoholysis. Functional data obtained with the processing assay indicate that the central region of the protein is responsible for viral DNA specificity. Target site selection for nonspecific alcoholysis again mapped to the central domain of IN, confirming our previous data indicating that this region can position nonviral DNA for nucleophilic attack. However, the chimeric proteins created patterns of viral DNA insertion distinct from that of either wild-type IN, suggesting that interactions between regions of IN influence target site selection for viral DNA integration. The results support a new model for the functional organization of IN in which viral DNA initially binds nonspecifically to the C-terminal portion of IN but the catalytic central region of the enzyme has a prominent role both in specific recognition of viral DNA ends and in positioning the host DNA for viral DNA integration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.