ArticleVirus research1997
Generation of cytopathogenic subgenomic RNA of classical swine fever virus in persistently infected porcine cell lines.
Article in Virus research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- Evolution of Porcine Virus Isolation: Guidelines for Practical Laboratory Application.Microorganisms · 2025Review
- Evolutionary-Related High- and Low-Virulent Classical Swine Fever Virus Isolates Reveal Viral Determinants of Virulence.Viruses · 2024Article
- TNF-Mediated Inhibition of Classical Swine Fever Virus Replication Is IRF1-, NF-κB- and JAK/STAT Signaling-Dependent.Viruses · 2021Article
- Classical swine fever virus can remain virulent after specific elimination of the interferon regulatory factor 3-degrading function of Npro.Journal of virology · 2009Article
- Analysis of hepatitis C virus superinfection exclusion by using novel fluorochrome gene-tagged viral genomes.Journal of virology · 2007Article
- Genome comparison of a novel classical swine fever virus isolated in China in 2004 with other CSFV strains.Virus genes · 2006Article
- Dual mechanisms of pestiviral superinfection exclusion at entry and RNA replication.Journal of virology · 2005Article
- Characterization of helper virus-independent cytopathogenic classical swine fever virus generated by an in vivo RNA recombination system.Journal of virology · 2005Article
- Classical swine fever virus interferes with cellular antiviral defense: evidence for a novel function of N(pro).Journal of virology · 2003Article
- Nucleocapsid protein-based enzyme-linked immunosorbent assay for detection and differentiation of antibodies against European and North American porcine reproductive and respiratory syndrome virus.Clinical and diagnostic laboratory immunology · 2002Article
- RNA recombination between persisting pestivirus and a vaccine strain: generation of cytopathogenic virus and induction of lethal disease.Journal of virology · 2001Article
- Efficient translation initiation is required for replication of bovine viral diarrhea virus subgenomic replicons.Journal of virology · 2001Article
- Generation and characterization of a hepatitis C virus NS3 protease-dependent bovine viral diarrhea virus.Journal of virology · 2000Article
- Classical swine fever virus E(rns) deletion mutants: trans-complementation and potential use as nontransmissible, modified, live-attenuated marker vaccines.Journal of virology · 2000Article
- Cytopathogenic and noncytopathogenic RNA replicons of classical swine fever virus.Journal of virology · 1999Article
- Classical swine fever virus leader proteinase Npro is not required for viral replication in cell culture.Journal of virology · 1998Article
- A recombinant classical swine fever virus stably expresses a marker gene.Journal of virology · 1998Article
- Infectious bovine viral diarrhea virus (strain NADL) RNA from stable cDNA clones: a cellular insert determines NS3 production and viral cytopathogenicity.Journal of virology · 1998Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two biological clones (A.1 and B.2) of the classical swine fever virus strain Alfort/187 and the recombinant virus vA187-1, derived from a cDNA clone of Alfort/187, were used to establish persistently infected cultures of the swine kidney cell lines SK-6 and PK-41. It was found that 100% of the cells in the passaged cultures were positive for viral antigen throughout the course of the experiment. Additionally, supernatants collected upon passaging of the cells continuously contained high titers of infectious virus. In six separate cultures persistently infected with either the biological clones or the recombinant virus, a cytopathic effect occurred spontaneously between passage 8 and 94. The cytopathogenic agent in the supernatants of these cultures could be passaged repeatedly, suggesting the generation of a mutant virus. Analysis of RNA from such cultures revealed the presence of a subgenomic viral RNA of approximately 8 kilobases (kb). In all six cases, this RNA had an identical internal deletion of 4764 nucleotides, including the region coding for all structural proteins. The subgenomic RNA replicated and was packaged in the presence of wild-type virus. Cells infected with cytopathogenic virus contained increased amounts of the viral protein NS3 thought to be involved in pestivirus cytopathogenicity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.