ArticleJournal of bacteriology1998
Variation in flagellin genes and proteins of Burkholderia cepacia.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
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- Contribution of Burkholderia cenocepacia flagella to infectivity and inflammation.Infection and immunity · 2004Article
- Specific and sensitive detection of Ralstonia solanacearum in soil on the basis of PCR amplification of fliC fragments.Applied and environmental microbiology · 2003Article
- Molecular typing of, and distribution of genetic markers among, Burkholderia cepacia complex isolates from Brazil.Journal of clinical microbiology · 2003Article
- Role of flagella in host cell invasion by Burkholderia cepacia.Infection and immunity · 2002Article
- Mutational analysis of the Rhizobium lupini H13-3 and Sinorhizobium meliloti flagellin genes: importance of flagellin A for flagellar filament structure and transcriptional regulation.Journal of bacteriology · 2001Article
- Phenotypic and genotypic diversity of the flagellin gene (fliC) among Clostridium difficile isolates from different serogroups.Journal of clinical microbiology · 2000Article
- Distinguishing species of the Burkholderia cepacia complex and Burkholderia gladioli by automated ribotyping.Journal of clinical microbiology · 2000Article
- Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara(-) phenotypes.Journal of clinical microbiology · 2000Article
- Pseudomonas and all that.Archives of disease in childhood · 1999Article
- Identification of Burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The majority of isolates of Burkholderia cepacia, an important opportunistic pathogen associated with cystic fibrosis, can be classified into two types on the basis of flagellin protein size. Electron microscopic analysis indicates that the flagella of strains with the larger flagellin type (type I) are wider in diameter. Flagellin genes representative of both types were cloned and sequenced to design oligonucleotide primers for PCR amplification of the central variable domain of B. cepacia flagellin genes. PCR-restriction fragment length polymorphism analysis of amplified B. cepacia flagellin gene products from 16 strains enabled flagellin type classification on the basis of product size and revealed considerable differences in sequence, indicating that the flagellin gene is a useful biomarker for epidemiological and phylogenetic studies of this organism.
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