ArticleGene expression1997
C/EBP proteins contain nuclear localization signals imbedded in their basic regions.
Article in Gene expression, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 60 citations in OpenAlex.
- Chronic low-dose of BPS and PFOS alter adipogenic programming and impair insulin responsiveness in human adipocytes.Journal of endocrinological investigation · 2026Article
- Genotype-phenotype correlations in specific granule deficiency: loss of DNA-binding ability and impaired nuclear localization cause severe manifestations due to the c.655_665del CEBPE variant.Clinical and experimental immunology · 2025Article
- CDK6 inhibits de novo lipogenesis in white adipose tissues but not in the liver.Nature communications · 2024Article
- CCAAT/Enhancer-Binding Proteins in Fibrosis: Complex Roles Beyond Conventional Understanding.Research (Washington, D.C.) · 2022Review
- KPT-330 Prevents Aortic Valve Calcification via a Novel C/EBPβ Signaling Pathway.Circulation research · 2021Article
- Phosphorylation within the bipartite NLS alters the localization and toxicity of the ER stress response factor DDIT3/CHOP.Cellular signalling · 2020Article
- C/EBPα in normal and malignant myelopoiesis.International journal of hematology · 2015Review
- Article
- Ubiquitin tagged dominant negative induces degradation of B-ZIP proteins.Biochemical and biophysical research communications · 2012Article
- CCAAT/enhancer binding protein delta (C/EBPdelta, CEBPD)-mediated nuclear import of FANCD2 by IPO4 augments cellular response to DNA damage.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The arylstibonic acid compound NSC13746 disrupts B-ZIP binding to DNA in living cells.European journal of cell biology · 2010Article
- Nucleolar retention of a translational C/EBPalpha isoform stimulates rDNA transcription and cell size.The EMBO journal · 2010Article
- The importance of being flexible: the case of basic region leucine zipper transcriptional regulators.Current protein & peptide science · 2009Review
- Induction of sodium iodide symporter gene and molecular characterisation of HNF3 beta/FoxA2, TTF-1 and C/EBP beta in thyroid carcinoma cells.British journal of cancer · 2008Article
- Differential gene regulation by selective association of transcriptional coactivators and bZIP DNA-binding domains.Molecular and cellular biology · 2006Article
- p27(SJ), a novel protein in St John's Wort, that suppresses expression of HIV-1 genome.Gene therapy · 2006Article
- Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha in the living pituitary cell nucleus.Molecular endocrinology (Baltimore, Md.) · 2003Article
- Interaction of HCF-1 with a cellular nuclear export factor.The Journal of biological chemistry · 2002Article
- A distinct bipartite motif is required for the localization of inhibitory kappaB-like (IkappaBL) protein to nuclear speckles.The Biochemical journal · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The C/EBP-related proteins (C/EBPalpha, CRP1, C/EBPbeta, and C/EBPdelta) form a subfamily of bZIP (basic region/leucine zipper) transcription factors that display sequence homology within the bZIP domain. The conserved basic region contains two motifs that exhibit significant homology to the bipartite nuclear localization signal (NLS) first described in nucleoplasmin. CRP1 and C/EBPbeta proteins bearing deletions of the basic region accumulate in the cytoplasm, in contrast to their normal nuclear location. Analysis of chimeric proteins consisting of CRP1 basic region sequences fused to beta-galactosidase revealed that the CRP1 basic region contains a single NLS that differs from conventional bipartite signals in two ways. First, mutation of a pair of arginine residues at the N-terminus of the proposed NLS does not disrupt its function. Second, the CRP1 NLS requires additional nonbasic residues at its C-terminus. A basic residue within the CRP1 NLS that is not conserved within the C/EBP family is occupied instead by an uncharged residue in C/EBPalpha and C/EBPbeta. When this nonconserved arginine residue was changed to alanine the CRP1 NLS behaved as a classical bipartite signal, suggesting that bipartite NLSs are present in all family members but that NLSs of the individual members differ slightly. Additionally, mutation of critical NLS residues in the intact CRP1 and C/EBPbeta proteins showed that these elements exhibit more bipartite-like characteristics when present in their normal sequence context. Finally, we observed that a C/EBPbeta protein lacking its NLS can be localized to the nucleus when coexpressed with C/EBPalpha, indicating that a single NLS is sufficient to promote nuclear transport of a bZIP dimer.
Indexed as
Identifiers
9495318PMC6148256W131517112What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.