ArticleThe Biochemical journal1998
Recombinant expression and biochemical characterization of an NADPH:flavin oxidoreductase from Entamoeba histolytica.
Article in The Biochemical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 70 citations in OpenAlex.
- Diversity and Plasticity of Virulent Characteristics ofTropical medicine and infectious disease · 2023Review
- Monocyte dysregulation: consequences for hepatic infections.Seminars in immunopathology · 2021Review
- Article
- The oxygen reduction pathway and heat shock stress response are both required for Entamoeba histolytica pathogenicity.Current genetics · 2016Review
- A review of the proposed role of neutrophils in rodent amebic liver abscess models.Parasite (Paris, France) · 2016Review
- New drug target in protozoan parasites: the role of thioredoxin reductase.Frontiers in microbiology · 2015Review
- Immune Evasion Mechanisms of Entamoeba histolytica: Progression to Disease.Frontiers in microbiology · 2015Review
- A detoxifying oxygen reductase in the anaerobic protozoan Entamoeba histolytica.Eukaryotic cell · 2012Article
- Involvement of thermoplasmaquinone-7 in transplasma membrane electron transport of Entamoeba histolytica trophozoites: a key molecule for future rational chemotherapeutic drug designing.Journal of bioenergetics and biomembranes · 2011Article
- Dihydrolipoamide dehydrogenases of Advenella mimigardefordensis and Ralstonia eutropha catalyze cleavage of 3,3'-dithiodipropionic acid into 3-mercaptopropionic acid.Applied and environmental microbiology · 2010Article
- Host-microbe interactions and defense mechanisms in the development of amoebic liver abscesses.Clinical microbiology reviews · 2009Review
- Crosstalk at the initial encounter: interplay between host defense and ameba survival strategies.Current opinion in immunology · 2007Review
- Nitroimidazole action in Entamoeba histolytica: a central role for thioredoxin reductase.PLoS biology · 2007Article
- The 29-kilodalton thiol-dependent peroxidase of Entamoeba histolytica is a factor involved in pathogenesis and survival of the parasite during oxidative stress.Eukaryotic cell · 2007Article
- Current therapeutics, their problems, and sulfur-containing-amino-acid metabolism as a novel target against infections by "amitochondriate" protozoan parasites.Clinical microbiology reviews · 2007Review
- Pathogenesis of intestinal amebiasis: from molecules to disease.Clinical microbiology reviews · 2000Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene encoding a putative NADPH:flavin oxidoreductase of the protozoan parasite Entamoeba histolytica (Eh34) was recombinantly expressed in Escherichia coli. The purified recombinant protein (recEh34) has a molecular mass of about 35 kDa upon SDS/PAGE analysis, exhibits a flavoprotein-like absorption spectrum and contains 1 mol of non-covalently bound FMN per mol of protein. RecEh34 reveals two different enzymic activities. It catalyses the NADPH-dependent reduction of oxygen to hydrogen peroxide (H2O2), as well as of disulphides such as 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) and cystine. The disulphide reductase but not the H2O2-forming NADPH oxidase activity is inhibitable by sulphydryl-active compounds, indicating that a thiol component is part of the active site for the disulphide reductase activity, whereas for the H2O2-forming NADPH oxidase activity only the flavin is required. Compared with the recombinant protein, similar activities are present in amoebic extracts. Native Eh34 is active in a monomeric as well as in a dimeric state. In contrast to recEh34, no flavin was associated with the native protein. However, both NADPH oxidase as well as DTNB reductase activity were found to be dependent on the addition of FAD or FMN.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.