ArticleThe Journal of physiology1998
Activation of volume-regulated chloride currents by reduction of intracellular ionic strength in bovine endothelial cells.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 120 citations in OpenAlex.
- Recent advances in the structure, function and regulation of the volume-regulated anion channels and their role in immunity.The Journal of physiology · 2025Review
- Physiology of the volume-sensitive/regulatory anion channel VSOR/VRAC: part 2: its activation mechanisms and essential roles in organic signal release.The journal of physiological sciences : JPS · 2024Review
- Physiological Functions of the Volume-Regulated Anion Channel VRAC/LRRC8 and the Proton-Activated Chloride Channel ASOR/TMEM206.Handbook of experimental pharmacology · 2024Article
- Structure-function relationships of the LRRC8 subunits and subdomains of the volume-regulated anion channel (VRAC).Frontiers in cellular neuroscience · 2022Review
- Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains.Nature communications · 2021Article
- TRPM7 is an essential regulator for volume-sensitive outwardly rectifying anion channel.Communications biology · 2021Article
- LRRC8A homohexameric channels poorly recapitulate VRAC regulation and pharmacology.American journal of physiology. Cell physiology · 2021Article
- LRRC8 family proteins within lysosomes regulate cellular osmoregulation and enhance cell survival to multiple physiological stresses.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- The Volume-Regulated Anion Channel in Glioblastoma.Cancers · 2019Review
- A 30-year journey from volume-regulated anion currents to molecular structure of the LRRC8 channel.The Journal of general physiology · 2019Review
- Inhibition of human N- and T-type calcium channels by an ortho-phenoxyanilide derivative, MONIRO-1.British journal of pharmacology · 2018Article
- Article
- LRRC8/VRAC anion channels enhance β-cell glucose sensing and insulin secretion.Nature communications · 2018Article
- Hypotonic Challenge of Endothelial Cells Increases Membrane Stiffness with No Effect on Tether Force.Biophysical journal · 2018Article
- Subunit-dependent oxidative stress sensitivity of LRRC8 volume-regulated anion channels.The Journal of physiology · 2017Article
- Comparative Effects of Chloride Channel Inhibitors on LRRC8/VRAC-Mediated Chloride Conductance.Frontiers in pharmacology · 2017Article
- The LRRC8A Mediated "Swell Activated" Chloride Conductance Is Dispensable for Vacuolar Homeostasis in Neutrophils.Frontiers in pharmacology · 2017Article
- Ion channels in regulated cell death.Cellular and molecular life sciences : CMLS · 2016Review
- Non-essential contribution of LRRC8A to volume regulation.Pflugers Archiv : European journal of physiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. We have studied the effects of intracellular ionic strength (gamma 1) on the swelling-activated whole-cell Cl- current (ICl,swell) in cultured calf pulmonary artery endothelial cells (CPAE cells). 2. Reducing gamma 1 from 155 to 95 mM at constant osmolarity and Cl- concentration activates an outwardly rectifying current that is mainly carried by Cl- ions and inactivates at positive potentials. The amplitude of the current is larger at more reduced levels of gamma 1. 3. The permeability ratio for the anions I-, Br-, Cl- and gluconate (PI: PBr: PCl: Pgluc) was 1.35:1.03:1:0.17. 4. Blockers of the swelling-activated Cl- current in CPAE cells also inhibit the current which is activated by a reduction in gamma 1 with an IC50 of 1.1 microM for tamoxifen, 1.3 microM for mibefradil, and 35 microM for quinidine. 5. The protein tyrosine kinase inhibitors tyrphostin B46 (50 microM) and genistein (100 microM), which inhibit ICl,swell in CPAE cells, also inhibited the gamma 1-induced current by 92.9 +/- 2.4% (n = 3) and 41.2 +/- 5.0% (n = 4), respectively. 6. Hypertonic extracellular solutions rapidly and reversibly antagonized the gamma 1-activated current, whereas increasing gamma 1 from 155 to 195 mM precluded activation of ICl,swell by hypotonic shock. 7. It is concluded that a reduction of gamma 1 activates an anion current that is identical to that activated by cell swelling. Changes in intracellular ionic strength may shift the volume set point for activation of ICl,swell.
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