ArticleNucleic acids research1998
Parallel thermodynamic analysis of duplexes on oligodeoxyribonucleotide microchips.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Design and Validation of Specific Oligonucleotide Probes on Planar Magnetic Biosensors.Analytical chemistry · 2024Article
- Multiplex Assay for Rapid Detection and Analysis of Nucleic Acid Using Barcode Receptor Encoded Particle (BREP).Biomedicines · 2022Article
- Low-Cost Platform for Multiplexed Electrochemical Melting Curve Analysis.ACS measurement science au · 2022Article
- In-gel fluorescence detection by DNA polymerase elongation.APL bioengineering · 2020Article
- Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels.Polymers · 2020Article
- Single Nucleotide Polymorphism Genotyping in Single-Molecule Electronic Circuits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Magnetoresistive sensors for measurements of DNA hybridization kinetics - effect of TINA modifications.Scientific reports · 2017Article
- Hydrogel microparticles for biosensing.European polymer journal · 2015Article
- Oil-isolated hydrogel microstructures for sensitive bioassays on-chip.Analytical chemistry · 2013Article
- Responsive DNA-based hydrogels and their applications.Macromolecular rapid communications · 2013Review
- Bar-coded hydrogel microparticles for protein detection: synthesis, assay and scanning.Nature protocols · 2011Article
- Ultrasensitive multiplexed microRNA quantification on encoded gel microparticles using rolling circle amplification.Analytical chemistry · 2011Article
- Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study.Nucleic acids research · 2010Article
- Salt concentration effects on equilibrium melting curves from DNA microarrays.Biophysical journal · 2010Article
- Fabrication of uniform DNA-conjugated hydrogel microparticles via replica molding for facile nucleic acid hybridization assays.Analytical chemistry · 2010Article
- Search for genetic markers and functional variants involved in the development of opiate and cocaine addiction and treatment.Annals of the New York Academy of Sciences · 2010Review
- Optimization of encoded hydrogel particles for nucleic acid quantification.Analytical chemistry · 2009Article
- The effects of mismatches on hybridization in DNA microarrays: determination of nearest neighbor parameters.Nucleic acids research · 2009Article
- Model-based probe set optimization for high-performance microarrays.Nucleic acids research · 2009Article
- Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A microchip method has been developed for massive and parallel thermodynamic analyses of DNA duplexes. Fluorescently labeled oligonucleotides were hybridized with oligonucleotides immobilized in the 100 x 100 x 20 mum gel pads of the microchips. The equilibrium melting curves for all microchip duplexes were measured in real time in parallel for all microchip duplexes. Thermodynamic data for perfect and mismatched duplexes that were obtained using the microchip method directly correlated with data obtained in solution. Fluorescent labels or longer linkers between the gel and the oligonucleotides appeared to have no significant effect on duplex stability. Extending the immobilized oligonucleotides with a four-base mixture from the 3'-end or one or two universal bases (5-nitroindole) from the 3'- and/or 5'-end increased the stabilities of their duplexes. These extensions were applied to increase the stabilities of the duplexes formed with short oligonucleotides in microchips, to significantly lessen the differences in melting curves of the AT- and GC-rich duplexes, and to improve discrimination of perfect duplexes from those containing poorly recognized terminal mismatches. This study explored a way to increase the efficiency of sequencing by hybridization on oligonucleotide microchips.
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