ArticleNucleic acids research1998
Survey of four different photoreactive moieties for DNA photoaffinity labeling of yeast RNA polymerase III transcription complexes.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Development of Low Temperature Activatable Aryl Azide Adhesion Promoters as Versatile Surface Modifiers.ACS applied engineering materials · 2025Article
- Kinase-catalyzed crosslinking: A comparison of ATP-crosslinker analogs.Bioorganic & medicinal chemistry letters · 2024Article
- Photo-caged 2-butene-1,4-dial as an efficient, target-specific photo-crosslinker for covalent trapping of DNA-binding proteins.Chemical science · 2023Article
- Genetically encoded photochemical covalent crosslinking within the Hcp-1 self-assembling bacterial secretion machinery.Amino acids · 2018Article
- Probing the orientation of inhibitor and epoxy-eicosatrienoic acid binding in the active site of soluble epoxide hydrolase.Archives of biochemistry and biophysics · 2017Article
- Development of Sulfonamide Photoaffinity Inhibitors for Probing Cellular γ-Secretase.ACS chemical neuroscience · 2016Article
- Ty1 Integrase Interacts with RNA Polymerase III-specific Subcomplexes to Promote Insertion of Ty1 Elements Upstream of Polymerase (Pol) III-transcribed Genes.The Journal of biological chemistry · 2016Article
- 3-Trifluoromethyl-3-aryldiazirine photolabels with enhanced ambient light stability.Chemical communications (Cambridge, England) · 2016Article
- Identification of Subunit Binding Positions on a Model Fork and Displacements That Occur during Sequential Assembly of the Escherichia coli Primosome.The Journal of biological chemistry · 2015Article
- 1,3-Dipolar cycloaddition reactivities of perfluorinated aryl azides with enamines and strained dipolarophiles.Journal of the American Chemical Society · 2015Article
- Developing diazirine-based chemical probes to identify histone modification 'readers' and 'erasers'.Chemical science · 2015Article
- Photoaffinity labeling of transcription factors by DNA-templated crosslinking.Chemical science · 2015Article
- Analysis of amyloid nanostructures using photo-cross-linking: in situ comparison of three widely used photo-cross-linkers.ACS chemical biology · 2014Article
- A comparative study of ATP analogs for phosphorylation-dependent kinase-substrate crosslinking.Bioorganic & medicinal chemistry · 2014Article
- Mapping the protein interaction network for TFIIB-related factor Brf1 in the RNA polymerase III preinitiation complex.Molecular and cellular biology · 2014Article
- Structure of the human angiotensin II type 1 (AT1) receptor bound to angiotensin II from multiple chemoselective photoprobe contacts reveals a unique peptide binding mode.The Journal of biological chemistry · 2013Article
- Disparity in the DNA translocase domains of SWI/SNF and ISW2.Nucleic acids research · 2012Article
- The rate of polymerase release upon filling the gap between Okazaki fragments is inadequate to support cycling during lagging strand synthesis.Journal of molecular biology · 2011Article
- Bioorthogonal labeling of 5-hydroxymethylcytosine in genomic DNA and diazirine-based DNA photo-cross-linking probes.Accounts of chemical research · 2011Article
- Photo-cross-linkers incorporated into G-protein-coupled receptors in mammalian cells: a ligand comparison.Angewandte Chemie (International ed. in English) · 2011Article
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3 authors.
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Abstract
In order to optimize the detection of protein-DNA contacts by DNA photoaffinity labeling, we attached four different photoreactive groups to DNA and examined their ability to crosslink yeast RNA polymerase III (Pol III) transcription complexes. Photoreactive nucleotides containing an aryl azide (AB-dUMP), benzophenone (BP-dUMP), perfluorinated aryl azide (FAB-dUMP) or diazirine (DB-dUMP) coupled to 5-aminoallyl deoxyuridine were incorporated into the SUP4 tRNATyr gene at bp -3/-2 or +11. Photo-crosslinking with diazirine revealed contacts of Pol III with DNA that are not detected by DNA photoaffinity labeling using an aryl azide, fluorinated aryl azide or benzophenone group attached to DNA. These novel contacts were of the 82 kDa subunit of Pol III with DNA at bp -3/-2 in the initiation complex and of the 82, 40(37) and 31 kDa subunits of Pol III with DNA at bp +11 in elongation complexes stalled at bp +17. These results provide evidence for the subcomplex of the 82, 34 and 31 kDa subunits of Pol III being positioned near the transcription bubble of actively transcribing Pol III, as all three proteins were crosslinked at bp +11 of the stalled transcription complex.
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