ArticlePlant physiology1998
Calcium-Dependent Protein Phosphorylation May Mediate the Gibberellic Acid Response in Barley Aleurone
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 59 citations in OpenAlex.
- Late-maturity α-amylase (LMA): exploring the underlying mechanisms and end-use quality effects in wheat.Planta · 2021Review
- Differentiation mechanism and function of the cereal aleurone cells and hormone effects on them.Plant cell reports · 2014Review
- Calcium-dependent protein kinase isoforms in Petunia have distinct functions in pollen tube growth, including regulating polarity.The Plant cell · 2006Article
- A gibberellin-regulated calcineurin B in rice localizes to the tonoplast and is implicated in vacuole function.Plant physiology · 2005Article
- Identification of phosphoproteins regulated by gibberellin in rice leaf sheath.Plant molecular biology · 2005Article
- Article
- Regulation of CDPK isoforms during tuber development.Plant molecular biology · 2003Article
- An Arabidopsis calcium-dependent protein kinase is associated with the endoplasmic reticulum.Plant physiology · 2002Article
- A rice membrane-bound calcium-dependent protein kinase is activated in response to low temperature.Plant physiology · 2001Article
- Programmed cell death in cereal aleurone.Plant molecular biology · 2000Review
- Spatio-temporal accumulation and activity of calcium-dependent protein kinases during embryogenesis, seed development, and germination in sandalwood.Plant physiology · 2000Article
- Expression of a gibberellin-induced leucine-rich repeat receptor-like protein kinase in deepwater rice and its interaction with kinase-associated protein phosphatase.Plant physiology · 1999Article
- Relocation of a Ca2+-dependent protein kinase activity during pollen tube reorientationThe Plant cell · 1998Article
- Abscisic acid signal transduction in the barley aleurone is mediated by phospholipase D activity.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peptide substrates of well-defined protein kinases were microinjected into aleurone protoplasts of barley (Hordeum vulgare L. cv Himalaya) to inhibit, and therefore identify, protein kinase-regulated events in the transduction of the gibberellin (GA) and abscisic acid signals. Syntide-2, a substrate designed for Ca2+- and calmodulin (CaM)-dependent kinases, selectively inhibited the GA response, leaving constitutive and abscisic acid-regulated events unaffected. Microinjection of syntide did not affect the GA-induced increase in cytosolic [Ca2+], suggesting that it inhibited GA action downstream of the Ca2+ signal. When photoaffinity-labeled syntide-2 was electroporated into protoplasts and cross-linked to interacting proteins in situ, it selectively labeled proteins of approximately 30 and 55 kD. A 54-kD, soluble syntide-2 phosphorylating protein kinase was detected in aleurone cells. This kinase was activated by Ca2+ and was CaM independent, but was inhibited by the CaM antagonist N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide (250 mum), suggesting that it was a CaM-domain protein kinase-like activity. These results suggest that syntide-2 inhibits the GA response of the aleurone via an interaction with this kinase, implicating the 54-kD kinase as a Ca2+-dependent regulator of the GA response in these cells.
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Registered trials
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