ArticleThe Journal of cell biology1998
Corequirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
74 citing papers in PubMed, 219 citations in OpenAlex.
- Cardiac mechanotransduction from development to disease.APL bioengineering · 2026Review
- The biochemical mechanism of Rho GTPase membrane binding, activation and retention in activity patterning.The EMBO journal · 2025Article
- Self-assembly of CIP4 drives actin-mediated asymmetric pit-closing in clathrin-mediated endocytosis.Nature communications · 2023Article
- Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation.The Journal of cell biology · 2021Article
- A conserved PI(4,5)P2-binding domain is critical for immune regulatory function of DOCK8.Life science alliance · 2021Article
- Stochastic geometry sensing and polarization in a lipid kinase-phosphatase competitive reaction.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Lateral distribution of phosphatidylinositol 4,5-bisphosphate in membranes regulates formin- and ARP2/3-mediated actin nucleation.The Journal of biological chemistry · 2019Article
- Regulation of actin assembly by PI(4,5)P2 and other inositol phospholipids: An update on possible mechanisms.Biochemical and biophysical research communications · 2018Review
- Article
- Cecal MicroRNAome response to Salmonella enterica serovar Enteritidis infection in White Leghorn Layer.BMC genomics · 2017Article
- Investigation of the Interaction between Cdc42 and Its Effector TOCA1: HANDOVER OF Cdc42 TO THE ACTIN REGULATOR N-WASP IS FACILITATED BY DIFFERENTIAL BINDING AFFINITIES.The Journal of biological chemistry · 2016Article
- Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4.Oncotarget · 2016Article
- Small-molecule high-throughput screening utilizing Xenopus egg extract.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Cell-cycle regulation of formin-mediated actin cable assembly.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Phosphoinositides and membrane curvature switch the mode of actin polymerization via selective recruitment of toca-1 and Snx9.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions.Cell research · 2012Article
- Screening for small molecule inhibitors of embryonic pathways: sometimes you gotta crack a few eggs.Bioorganic & medicinal chemistry · 2012Review
- A biochemical screen for identification of small-molecule regulators of the Wnt pathway using Xenopus egg extracts.Journal of biomolecular screening · 2011Article
- WAVE regulatory complex activation by cooperating GTPases Arf and Rac1.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Both phosphoinositides and small GTP-binding proteins of the Rho family have been postulated to regulate actin assembly in cells. We have reconstituted actin assembly in response to these signals in Xenopus extracts and examined the relationship of these pathways. We have found that GTPgammaS stimulates actin assembly in the presence of endogenous membrane vesicles in low speed extracts. These membrane vesicles are required, but can be replaced by lipid vesicles prepared from purified phospholipids containing phosphoinositides. Vesicles containing phosphatidylinositol (4,5) bisphosphate or phosphatidylinositol (3,4,5) trisphosphate can induce actin assembly even in the absence of GTPgammaS. RhoGDI, a guanine-nucleotide dissociation inhibitor for the Rho family, inhibits phosphoinositide-induced actin assembly, suggesting the involvement of the Rho family small G proteins. Using various dominant mutants of these G proteins, we demonstrate the requirement of Cdc42 for phosphoinositide-induced actin assembly. Our results suggest that phosphoinositides may act to facilitate GTP exchange on Cdc42, as well as to anchor Cdc42 and actin nucleation activities. Hence, both phosphoinositides and Cdc42 are required to induce actin assembly in this cell-free system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.