ArticlePlant physiology1998
Molecular cloning of vacuolar H(+)-pyrophosphatase and its developmental expression in growing hypocotyl of mung bean.
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 94 citations in OpenAlex.
- Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants.Antioxidants (Basel, Switzerland) · 2024Review
- BnVP1, a novel vacuolar H+ pyrophosphatase gene from Boehmeria nivea confers cadmium tolerance in transgenic Arabidopsis.PloS one · 2024Article
- The flip side of theThe Journal of biological chemistry · 2019Article
- Characterization and expression analyses of the H⁺-pyrophosphatase gene in rye.Journal of genetics · 2016Article
- Apoplasmic loading in the rice phloem supported by the presence of sucrose synthase and plasma membrane-localized proton pyrophosphatase.Annals of botany · 2016Article
- Contribution of PPi-Hydrolyzing Function of Vacuolar H(+)-Pyrophosphatase in Vegetative Growth of Arabidopsis: Evidenced by Expression of Uncoupling Mutated Enzymes.Frontiers in plant science · 2016Article
- Comparative analysis of potassium deficiency-responsive transcriptomes in low potassium susceptible and tolerant wheat (Triticum aestivum L.).Scientific reports · 2015Article
- Dynamics of vacuoles and H+-pyrophosphatase visualized by monomeric green fluorescent protein in Arabidopsis: artifactual bulbs and native intravacuolar spherical structures.The Plant cell · 2014Article
- Physiological and molecular mechanisms of plant salt tolerance.Photosynthesis research · 2013Review
- Article
- Fe deficiency differentially affects the vacuolar proton pumps in cucumber and soybean roots.Frontiers in plant science · 2013Article
- Arabidopsis V-ATPase activity at the tonoplast is required for efficient nutrient storage but not for sodium accumulation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Purification and functional characterization of protoplasts and intact vacuoles from grape cells.BMC research notes · 2010Article
- Regulation by salt of vacuolar H+-ATPase and H+-pyrophosphatase activities and Na+/H+ exchange.Plant signaling & behavior · 2009Review
- Arbuscular mycorrhizal fungi induce differential activation of the plasma membrane and vacuolar H+ pumps in maize roots.Mycorrhiza · 2009Article
- Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity.The Journal of biological chemistry · 2008Article
- Sequence analysis and transcriptional profiling of two vacuolar H+ -pyrophosphatase isoforms in Vitis vinifera.Journal of plant research · 2006Article
- Molecular cloning and characterization of a vacuolar H+ -pyrophosphatase gene, SsVP, from the halophyte Suaeda salsa and its overexpression increases salt and drought tolerance of Arabidopsis.Plant molecular biology · 2006Article
- Importance of Rhodospirillum rubrum H(+)-pyrophosphatase under low-energy conditions.Journal of bacteriology · 2004Article
- Differential regulation of soluble and membrane-bound inorganic pyrophosphatases in the photosynthetic bacterium Rhodospirillum rubrum provides insights into pyrophosphate-based stress bioenergetics.Journal of bacteriology · 2004Article
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2 authors at 1 institution in 1 country.
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Abstract
Vacuolar proton-translocating inorganic pyrophosphatase and H(+)-ATPase acidify the vacuoles and power the vacuolar secondary active transport systems in plants. Developmental changes in the transcription of the pyrophosphatase in growing hypocotyls of mung bean (Vigna radiata) were investigated. The cDNA clone for the mung bean enzyme contains an uninterrupted open reading frame of 2298 bp, coding for a polypeptide of 766 amino acids. Hypocotyls were divided into elongating and mature regions. RNA analysis revealed that the transcript level of the pyrophosphatase was high in the elongating region of the 3-d-old hypocotyl but was extremely low in the mature region of the 5-d-old hypocotyl. The level of transcript of the 68-kD subunit of H(+)-ATPase also decreased after cell maturation. In the elongating region, the proton-pumping activity of pyrophosphatase on the basis of membrane protein was 3 times higher than that of H(+)-ATPase. After cell maturation, the pyrophosphatase activity decreased to 30% of that in the elongating region. The decline in the pyrophosphatase activity was in parallel with a decrease in the enzyme protein content. These findings indicate that the level of the pyrophosphatase, a main vacuolar proton pump in growing cells, is negatively regulated after cell maturation at the transcriptional level.
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