ArticleMolecular and cellular biology1998
Nonhomologous end joining during restriction enzyme-mediated DNA integration in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Pichia pastoris Random Mutagenesis and Screening.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii.Communications biology · 2022Article
- Requirement of POL3 and POL4 on non-homologous and microhomology-mediated end joining in rad50/xrs2 mutants of Saccharomyces cerevisiae.Mutagenesis · 2015Article
- PFP1, a gene encoding an Epc-N domain-containing protein, is essential for pathogenicity of the barley pathogen Rhynchosporium commune.Eukaryotic cell · 2014Article
- Effect of rad50 mutation on illegitimate recombination in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2011Article
- Formation of AAV single stranded DNA genome from a circular plasmid in Saccharomyces cerevisiae.PloS one · 2011Article
- Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Review
- Rad1, rad10 and rad52 mutations reduce the increase of microhomology length during radiation-induced microhomology-mediated illegitimate recombination in saccharomyces cerevisiae.Radiation research · 2009Article
- Ionizing radiation induces microhomology-mediated end joining in trans in yeast and mammalian cells.Radiation research · 2009Article
- High-throughput analysis of spatio-temporal dynamics in Dictyostelium.Genome biology · 2007Article
- Ionizing radiation and restriction enzymes induce microhomology-mediated illegitimate recombination in Saccharomyces cerevisiae.Nucleic acids research · 2007Article
- The Swi/Snf chromatin remodeling complex is required for ribosomal DNA and telomeric silencing in Saccharomyces cerevisiae.Molecular and cellular biology · 2004Article
- Cercosporin-deficient mutants by plasmid tagging in the asexual fungus Cercospora nicotianae.Molecular genetics and genomics : MGG · 2003Article
- Promoter-trapping in Saccharomyces cerevisiae by radiation-assisted fragment insertion.Nucleic acids research · 2002Article
- Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells.Nucleic acids research · 2001Article
- Article
- DNA integration by Ty integrase in yku70 mutant Saccharomyces cerevisiae cells.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The BamHI restriction enzyme mediates integration of nonhomologous DNA into the Saccharomyces cerevisiae genome (R. H. Schiestl and T. D. Petes, Proc. Natl. Acad. Sci. USA 88:7585-7589, 1991). The present study investigates the mechanism of such events: in particular, the mediating activity of various restriction enzymes and the processing of resultant fragment ends. Our results show that in addition to BamHI, BglII and KpnI increase DNA integration efficiencies severalfold, while Asp718, HindIII, EcoRI, SalI, SmaI, HpaI, MscI, and SnaBI do not. Secondly, the three active enzymes stimulated integrations only of fragments containing 5' or 3' overhangs but not of blunt-ended fragments. Thirdly, integrations mediated by one enzyme and utilizing a substrate created by another required at least 2 bp of homology. Furthermore, an Asp718 fragment possessing a 5' overhang integrated into a KpnI (isoschizomer) site possessing a 3' overhang, most likely by filling of the 5' overhang followed by 5' exonuclease digestion to produce a 3' end. We classified and analyzed the restriction enzyme-mediated integration events in the context of their genomic positions. The majority of events integrated into single sites. In the remaining 6 of 19 cases each end of the plasmid inserted into a different sequence, producing rearrangements such as duplications, deletions, and translocations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.