ArticleMolecular and cellular biology1998
Identification and topological arrangement of Drosophila proximal sequence element (PSE)-binding protein subunits that contact the PSEs of U1 and U6 small nuclear RNA genes.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed.
- Functional and evolutionary analysis of the Arabidopsis 4R-MYB protein SNAPc4 as part of the SNAP complex.Plant physiology · 2021Article
- Assembly of SNAPc, Bdp1, and TBP on the U6 snRNA Gene Promoter in Drosophila melanogaster.Molecular and cellular biology · 2020Article
- Transcriptional regulation of snRNAs and its significance for plant development.Journal of plant research · 2017Review
- The Myb domain of the largest subunit of SNAPc adopts different architectural configurations on U1 and U6 snRNA gene promoter sequences.Nucleic acids research · 2014Article
- Architectural arrangement of the small nuclear RNA (snRNA)-activating protein complex 190 subunit (SNAP190) on U1 snRNA gene promoter DNA.The Journal of biological chemistry · 2012Article
- Identification of SNAPc subunit domains that interact with specific nucleotide positions in the U1 and U6 gene promoters.Molecular and cellular biology · 2010Article
- A map of Drosophila melanogaster small nuclear RNA-activating protein complex (DmSNAPc) domains involved in subunit assembly and DNA binding.The Journal of biological chemistry · 2009Article
- Static and kinetic site-specific protein-DNA photocrosslinking: analysis of bacterial transcription initiation complexes.Methods in molecular biology (Clifton, N.J.) · 2009Article
- In vivo analysis of Caenorhabditis elegans noncoding RNA promoter motifs.BMC molecular biology · 2008Article
- Transcriptional regulation of human small nuclear RNA genes.Biochimica et biophysica acta · 2008Review
- Insect small nuclear RNA gene promoters evolve rapidly yet retain conserved features involved in determining promoter activity and RNA polymerase specificity.Nucleic acids research · 2007Article
- The PSEA promoter element of the Drosophila U1 snRNA gene is sufficient to bring DmSNAPc into contact with 20 base pairs of downstream DNA.Nucleic acids research · 2005Article
- Architectural arrangement of cloned proximal sequence element-binding protein subunits on Drosophila U1 and U6 snRNA gene promoters.Molecular and cellular biology · 2004Article
- Multiple, dispersed human U6 small nuclear RNA genes with varied transcriptional efficiencies.Nucleic acids research · 2003Article
- Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeast.Molecular and cellular biology · 2001Article
- Similarities and differences in the conformation of protein-DNA complexes at the U1 and U6 snRNA gene promoters.Nucleic acids research · 2000Article
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2 authors.
Funding
Abstract
Most small nuclear RNAs (snRNAs) are synthesized by RNA polymerase II, but U6 and a few others are synthesized by RNA polymerase III. Transcription of snRNA genes by either polymerase is dependent on a proximal sequence element (PSE) located upstream of position -40 relative to the transcription start site. In contrast to findings in vertebrates, sea urchins, and plants, the RNA polymerase specificity of Drosophila snRNA genes is intrinsically encoded in the PSE sequence itself. We have investigated the differential interaction of the Drosophila melanogaster PSE-binding protein (DmPBP) with U1 and U6 gene PSEs. By using a site specific protein-DNA photo-cross-linking assay, we identified three polypeptide subunits of DmPBP with apparent molecular masses of 95, 49, and 45 kDa that are in close proximity to the DNA and two additional putative polypeptides of 230 and 52 kDa that may be integral to the complex. The 95-kDa subunit cross-linked at positions spanning the entire length of the PSE, but the 49- and 45-kDa subunits cross-linked only to the 3' half of the PSE. The same polypeptides cross-linked to both the U1 and U6 PSE sequences. However, there were significant differences in the cross-linking patterns of these subunits at a subset of the phosphate positions, depending on whether binding was to a U1 or U6 gene PSE. These data suggest that RNA polymerase specificity is associated with distinct modes of interaction of DmPBP with the DNA at U1 and U6 promoters.
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