ArticleMolecular and cellular biology1998
The regulator of nitrate assimilation in ascomycetes is a dimer which binds a nonrepeated, asymmetrical sequence.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 58 citations in OpenAlex.
- Characterization of the promoter of the nitrate transporter-encoding gene nrtA in Aspergillus nidulans.Molecular genetics and genomics : MGG · 2020Article
- Nitrate assimilation pathway (NAP): role of structural (nit) and transporter (ntr1) genes in Fusarium oxysporum f.sp. lycopersici growth and pathogenicity.Current genetics · 2018Article
- Article
- Transcription Factors in Fungi: TFome Dynamics, Three Major Families, and Dual-Specificity TFs.Frontiers in genetics · 2017Article
- Nitrate Assimilation inFrontiers in microbiology · 2017Article
- Genomic and transcriptomic analyses of the tangerine pathotype of Alternaria alternata in response to oxidative stress.Scientific reports · 2016Article
- Nitric oxide in fungi: is there NO light at the end of the tunnel?Current genetics · 2016Review
- Nitric oxide synthesis by nitrate reductase is regulated during development in Aspergillus.Molecular microbiology · 2016Article
- Reversible Oxidation of a Conserved Methionine in the Nuclear Export Sequence Determines Subcellular Distribution and Activity of the Fungal Nitrate Regulator NirA.PLoS genetics · 2015Article
- Article
- Pseudo-constitutivity of nitrate-responsive genes in nitrate reductase mutants.Fungal genetics and biology : FG & B · 2013Article
- Transcriptome analysis of nitrate assimilation in Aspergillus nidulans reveals connections to nitric oxide metabolism.Molecular microbiology · 2010Article
- MlcR, a zinc cluster activator protein, is able to bind to a single (A/T)CGG site of cognate asymmetric motifs in the ML-236B (compactin) biosynthetic gene cluster.Molecular genetics and genomics : MGG · 2009Article
- A library-based method to rapidly analyse chromatin accessibility at multiple genomic regions.Nucleic acids research · 2009Article
- Nuclear export of the transcription factor NirA is a regulatory checkpoint for nitrate induction in Aspergillus nidulans.Molecular and cellular biology · 2007Article
- A fungal family of transcriptional regulators: the zinc cluster proteins.Microbiology and molecular biology reviews : MMBR · 2006Review
- Metabolically independent and accurately adjustable Aspergillus sp. expression system.Applied and environmental microbiology · 2005Article
- A paradoxical mutant GATA factor.Eukaryotic cell · 2004Article
- Chromatin rearrangements in the prnD-prnB bidirectional promoter: dependence on transcription factors.Eukaryotic cell · 2004Article
- Apparent genetic redundancy facilitates ecological plasticity for nitrate transport.The EMBO journal · 2001Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulation of nitrate assimilation seems to follow the same pattern in all ascomycetes where this process has been studied. We show here by in vitro binding studies and a number of protection and interference techniques that the transcription factor mediating nitrate induction in Aspergillus nidulans, a protein containing a binuclear zinc cluster DNA binding domain, recognizes an asymmetrical sequence of the form CTCC GHGG. We further show that the protein binds to its consensus site as a dimer. We establish the role of the putative dimerization element by its ability to replace the analogous element of the cI protein of phage lambda. Mutagenesis of crucial leucines of the dimerization element affect both the binding ability of the dimer and the conformation of the resulting protein-DNA complex. This is the first case to be described where a dimer recognizes such an asymmetrical nonrepeated sequence, presumably by each monomeric subunit making different contacts with different DNA half-sites.
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