ArticleMolecular and cellular biology1998
Bone-specific expression of the alpha chain of the nascent polypeptide-associated complex, a coactivator potentiating c-Jun-mediated transcription.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 88 citations in OpenAlex.
- Improving dermal fibroblast-to-epidermis communications and aging wound repair through extracellular vesicle-mediated delivery of Gstm2 mRNA.Journal of nanobiotechnology · 2024Article
- Nacα protects the larval fat body from cell death by maintaining cellular proteostasis in Drosophila.Nature communications · 2023Article
- Nascent polypeptide-Associated Complex and Signal Recognition Particle have cardiac-specific roles in heart development and remodeling.PLoS genetics · 2022Article
- NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH.International journal of molecular sciences · 2022Article
- Contribution of the Transcription Factors Sp1/Sp3 and AP-1 toInternational journal of molecular sciences · 2021Article
- Ubiquitin specific peptidase Usp53 regulates osteoblast versus adipocyte lineage commitment.Scientific reports · 2021Article
- Lnc-GD2H Promotes Proliferation by Forming a Feedback Loop With c-Myc and Enhances Differentiation Through Interacting With NACA to Upregulate Myog in C2C12 Myoblasts.Frontiers in cell and developmental biology · 2021Article
- Osj10gBTF3-Mediated Import of Chloroplast Protein Is Essential for Pollen Development in Rice.Frontiers in plant science · 2021Article
- The beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development ofInternational journal of molecular sciences · 2020Article
- BTF3 sustains cancer stem-like phenotype of prostate cancer via stabilization of BMI1.Journal of experimental & clinical cancer research : CR · 2019Article
- Dephosphorylation of the transcriptional cofactor NACA by the PP1A phosphatase enhances cJUN transcriptional activity and osteoblast differentiation.The Journal of biological chemistry · 2019Article
- NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation.Nature communications · 2015Article
- Deciphering the mystery of hepatitis B virus receptors: A historical perspective.Virusdisease · 2015Review
- The PTH-Gαs-protein kinase A cascade controls αNAC localization to regulate bone mass.Molecular and cellular biology · 2014Article
- The nascent polypeptide-associated complex is a key regulator of proteostasis.The EMBO journal · 2013Article
- Comparative analysis of the transcriptome in tissues secreting purple and white nacre in the pearl mussel Hyriopsis cumingii.PloS one · 2013Article
- Combinatorial control of ATF4-dependent gene transcription in osteoblasts.Annals of the New York Academy of Sciences · 2011Review
- Crystal structures of NAC domains of human nascent polypeptide-associated complex (NAC) and its αNAC subunit.Protein & cell · 2010Article
- Nuclear alpha NAC influences bone matrix mineralization and osteoblast maturation in vivo.Molecular and cellular biology · 2010Article
- FIAT is co-expressed with its dimerization target ATF4 in early osteoblasts, but not in osteocytes.Gene expression patterns : GEP · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The alpha chain of the nascent polypeptide-associated complex (alpha-NAC) coactivator was shown to potentiate the activity of the homodimeric c-Jun activator, while transcription mediated by the c-Fos/c-Jun heterodimer was unaffected. The use of deletion mutants in pull-down assays revealed that alpha-NAC interacted with amino acids 1 to 89 of the c-Jun protein and that the coactivator could interact with both the unphosphorylated and the serine 73-phosphorylated form of c-Jun. N-terminal-deleted c-Jun protein failed to interact with alpha-NAC in mammalian two-hybrid assays, while mutant c-Jun proteins lacking the leucine zipper or the basic domain retained interaction with alpha-NAC in vivo. Kinetics studies with purified c-Jun homodimer and recombinant alpha-NAC proteins allowed determination of the mechanism of coactivation by alpha-NAC: the coactivator stabilized the AP-1 complex formed by the c-Jun homodimer on its DNA recognition sequence through an eightfold reduction in the dissociation constant (kd) of the complex. This effect of alpha-NAC was specific, because alpha-NAC could not stabilize the interactions of JunB or Sp1 with their cognate binding sites. Interestingly, the expression of alpha-NAC was first detected at 14.5 to 15 days postconception, concomitantly with the onset of ossification during embryogenesis. The alpha-NAC protein was specifically expressed in differentiated osteoblasts at the centers of ossification. Thus, the alpha-NAC gene product exhibits the properties of a developmentally regulated, bone-specific transcriptional coactivator.
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