ArticleMolecular and cellular biology1998
The C terminus of the major yeast telomere binding protein Rap1p enhances telomere formation.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- A sharp Pif1-dependent threshold separates DNA double-strand breaks from critically short telomeres.eLife · 2017Article
- Endogenous Hot Spots of De Novo Telomere Addition in the Yeast Genome Contain Proximal Enhancers That Bind Cdc13.Molecular and cellular biology · 2016Article
- Binding of Multiple Rap1 Proteins Stimulates Chromosome Breakage Induction during DNA Replication.PLoS genetics · 2015Article
- The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae.eLife · 2015Article
- Article
- Making the most of a little: dosage effects in eukaryotic telomere length maintenance.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Review
- The number of vertebrate repeats can be regulated at yeast telomeres by Rap1-independent mechanisms.The EMBO journal · 2003Article
- Preferential maintenance of critically short telomeres in mammalian cells heterozygous for mTert.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Subcellular localization of the yeast proteome.Genes & development · 2002Article
- Telomere formation by rap1p binding site arrays reveals end-specific length regulation requirements and active telomeric recombination.Molecular and cellular biology · 2001Article
- Yeast telomerase appears to frequently copy the entire template in vivo.Nucleic acids research · 2001Article
- Varying the number of telomere-bound proteins does not alter telomere length in tel1Delta cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Developmentally regulated telomerase activity is correlated with chromosomal healing during chromatin diminution in Ascaris suum.Molecular and cellular biology · 1999Article
- The yeast telomere length counting machinery is sensitive to sequences at the telomere-nontelomere junction.Molecular and cellular biology · 1999Article
- Progressive Rearrangement of Telomeric Sequences Added to Both the ITR Ends of the Yeast Linear pGKL Plasmid.Biological procedures onlineArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The telomeres of most organisms consist of short repeated sequences that can be elongated by telomerase, a reverse transcriptase complex that contains its own RNA template for the synthesis of telomere repeats. In Saccharomyces cerevisiae, the RAP1 gene encodes the major telomere binding protein Rap1p. Here we use a quantitative telomere formation assay to demonstrate that Rap1p C termini can enhance telomere formation more than 30-fold when they are located at internal sites. This stimulation is distinct from protection from degradation. Enhancement of formation required the gene for telomerase RNA but not Sir1p, Sir2p, Sir3p, Sir4p, Tel1p, or the Rif1p binding site in the Raplp C terminus. Our data suggest that Rap1p C termini enhance telomere formation by attracting or increasing the activity of telomerase near telomeres. Earlier work suggests that Rap1p molecules at the chromosome terminus inhibit the elongation of long telomeres by blocking the access of telomerase. Our results suggest a model where a balance between internal Rap1p increasing telomerase activity and Rap1p at the termini of long telomeres controlling telomerase access maintains telomeres at a constant length.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.