ArticleMolecular and cellular biology1998
Gal4p-mediated chromatin remodeling depends on binding site position in nucleosomes but does not require DNA replication.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Beyond assembly: the increasing flexibility of single-molecule sequencing technology.Nature reviews. Genetics · 2023Review
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- Telomeric repeats act as nucleosome-disfavouring sequences in vivo.Nucleic acids research · 2014Article
- Multiplex mapping of chromatin accessibility and DNA methylation within targeted single molecules identifies epigenetic heterogeneity in neural stem cells and glioblastoma.Genome research · 2014Article
- DNA methyltransferase accessibility protocol for individual templates by deep sequencing.Methods in enzymology · 2012Article
- Simultaneous single-molecule mapping of protein-DNA interactions and DNA methylation by MAPit.Current protocols in molecular biology · 2011Article
- MethylViewer: computational analysis and editing for bisulfite sequencing and methyltransferase accessibility protocol for individual templates (MAPit) projects.Nucleic acids research · 2011Article
- Nucleosome-depleted regions in cell-cycle-regulated promoters ensure reliable gene expression in every cell cycle.Developmental cell · 2010Article
- Single-molecule analysis of chromatin: changing the view of genomes one molecule at a time.Journal of cellular biochemistry · 2008Review
- A nucleosome positioned by alpha2/Mcm1 prevents Hap1 activator binding in vivo.Biochemical and biophysical research communications · 2007Article
- Article
- Single-molecule and population probing of chromatin structure using DNA methyltransferases.Methods (San Diego, Calif.) · 2007Article
- Site-selective in vivo targeting of cytosine-5 DNA methylation by zinc-finger proteins.Nucleic acids research · 2003Article
- Targeted cytosine methylation for in vivo detection of protein-DNA interactions.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- GCN5 dependence of chromatin remodeling and transcriptional activation by the GAL4 and VP16 activation domains in budding yeast.Molecular and cellular biology · 2001Article
- Protein binding protects sites on stable episomes and in the chromosome from de novo methylation.Molecular and cellular biology · 2001Article
- Tup1p represses Mcm1p transcriptional activation and chromatin remodeling of an a-cell-specific gene.The EMBO journal · 2000Article
- Cell cycle-dependent binding of yeast heat shock factor to nucleosomes.Molecular and cellular biology · 2000Article
- Artificially recruited TATA-binding protein fails to remodel chromatin and does not activate three promoters that require chromatin remodeling.Molecular and cellular biology · 2000Article
- S-Phase progression mediates activation of a silenced gene in synthetic nuclei.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Biochemical studies have demonstrated decreased binding of various proteins to DNA in nucleosome cores as their cognate sites are moved from the edge of the nucleosome to the pseudodyad (center). However, to date no study has addressed whether this structural characteristic of nucleosomes modulates the function of a transcription factor in living cells, where processes of DNA replication and chromatin modification or remodeling could significantly affect factor binding. Using a sensitive, high-resolution methyltransferase assay, we have monitored the ability of Gal4p in vivo to interact with a nucleosome at positions that are known to be inaccessible in nucleosome cores in vitro. Gal4p efficiently bound a single cognate site (UASG) centered at 41 bp from the edge of a positioned nucleosome, perturbing chromatin structure and inducing transcription. DNA binding and chromatin perturbation accompanying this interaction also occurred in the presence of hydroxyurea, indicating that DNA replication is not necessary for Gal4p-mediated nucleosome disruption. These data extend previous studies, which demonstrated DNA replication-independent chromatin remodeling, by showing that a single dimer of Gal4p, without the benefit of cooperative interactions that occur at complex wild-type promoters, is competent for invasion of a preestablished nucleosome. When the UASG was localized at the nucleosomal pseudodyad, relative occupancy by Gal4p, nucleosome disruption, and transcriptional activation were substantially compromised. Therefore, despite the increased nucleosome binding capability of Gal4p in cells, the precise translational position of a factor binding site in one nucleosome in an array can affect the ability of a transcriptional regulator to overcome the repressive influence of chromatin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.