ArticleMolecular and cellular biology1998
Functional relationships of Srb10-Srb11 kinase, carboxy-terminal domain kinase CTDK-I, and transcriptional corepressor Ssn6-Tup1.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 1 of them a synthesis that pooled it.
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Who cites it
59 citing papers in PubMed, 1 synthesis or guideline pooled it, 126 citations in OpenAlex.
- Pooled it
- The transcriptional repressor Ssn6 modulates phase separation to regulate fungal gene expression.mBio · 2026Article
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1.Current genetics · 2023Article
- Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.Current genetics · 2022Article
- TORC1 signaling modulates Cdk8-dependent GAL gene expression in Saccharomyces cerevisiae.Genetics · 2021Article
- Genetic analysis argues for a coactivator function for the Saccharomyces cerevisiae Tup1 corepressor.Genetics · 2021Article
- FgSsn3 kinase, a component of the mediator complex, is important for sexual reproduction and pathogenesis in Fusarium graminearum.Scientific reports · 2016Article
- The Transcriptional Coregulator LEUNIG_HOMOLOG Inhibits Light-Dependent Seed Germination in Arabidopsis.The Plant cell · 2015Article
- Microevolution of Candida albicans in macrophages restores filamentation in a nonfilamentous mutant.PLoS genetics · 2014Article
- Analysis of Candida albicans mutants defective in the Cdk8 module of mediator reveal links between metabolism and biofilm formation.PLoS genetics · 2014Article
- Review
- Protein kinase A contributes to the negative control of Snf1 protein kinase in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- Distinct role of Mediator tail module in regulation of SAGA-dependent, TATA-containing genes in yeast.The EMBO journal · 2012Article
- The Cyc8-Tup1 complex inhibits transcription primarily by masking the activation domain of the recruiting protein.Genes & development · 2011Article
- The general transcriptional repressor Tup1 is required for dimorphism and virulence in a fungal plant pathogen.PLoS pathogens · 2011Article
- The metazoan Mediator co-activator complex as an integrative hub for transcriptional regulation.Nature reviews. Genetics · 2010Review
- Activator control of nucleosome occupancy in activation and repression of transcription.PLoS biology · 2008Article
- The transcription corepressor LEUNIG interacts with the histone deacetylase HDA19 and mediator components MED14 (SWP) and CDK8 (HEN3) to repress transcription.Molecular and cellular biology · 2007Article
- Individual subunits of the Ssn6-Tup11/12 corepressor are selectively required for repression of different target genes.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
The Srb10-Srb11 protein kinase of Saccharomyces cerevisiae is a cyclin-dependent kinase (cdk)-cyclin pair which has been found associated with the carboxy-terminal domain (CTD) of RNA polymerase II holoenzyme forms. Previous genetic findings implicated the Srb10-Srb11 kinase in transcriptional repression. Here we use synthetic promoters and LexA fusion proteins to test the requirement for Srb10-Srb11 in repression by Ssn6-Tup1, a global corepressor. We show that srb10delta and srb11delta mutations reduce repression by DNA-bound LexA-Ssn6 and LexA-Tup1. A point mutation in a conserved subdomain of the kinase similarly reduced repression, indicating that the catalytic activity is required. These findings establish a functional link between Ssn6-Tup1 and the Srb10-Srb11 kinase in vivo. We also explored the relationship between Srb10-Srb11 and CTD kinase I (CTDK-I), another member of the cdk-cyclin family that has been implicated in CTD phosphorylation. We show that mutation of CTK1, encoding the cdk subunit, causes defects in transcriptional repression by LexA-Tup1 and in transcriptional activation. Analysis of the mutant phenotypes and the genetic interactions of srb10delta and ctk1A suggests that the two kinases have related but distinct roles in transcriptional control. These genetic findings, together with previous biochemical evidence, suggest that one mechanism of repression by Ssn6-Tup1 involves functional interaction with RNA polymerase II holoenzyme.
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