ArticleClinical and experimental immunology1998
Abnormal intracellular IL-2 and interferon-gamma (IFN-gamma) production as HIV-1-assocated markers of immune dysfunction.
Article in Clinical and experimental immunology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
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- Epigenetic modulations in activated cells early after HIV-1 infection and their possible functional consequences.PloS one · 2015Article
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- Cytokine profiles in adult mink infected with Aleutian mink disease parvovirus.Journal of virology · 2003Article
- Decreases in plasma TNF-alpha level and IFN-gamma mRNA level in peripheral blood mononuclear cells (PBMC) and an increase in IL-2 mRNA level in PBMC are associated with effective highly active antiretroviral therapy in HIV-infected patients.Clinical and experimental immunology · 2003Article
- Longitudinal study of intracellular T cell cytokine production in infants compared to adults.Clinical and experimental immunology · 2002Article
- Differential modulation of surface and intracellular protein expression by T cells after stimulation in the presence of monensin or brefeldin A.Clinical and diagnostic laboratory immunology · 2002Article
- Comparison of serum and cell-specific cytokines in humans.Clinical and diagnostic laboratory immunology · 2001Article
- Chronic immune activation and inflammation as the cause of malignancy.British journal of cancer · 2001Review
- Immunological effects of interleukin-2 therapy in human immunodeficiency virus-positive subjects.Clinical and diagnostic laboratory immunology · 2001Review
- Enhanced interferon-gamma by CD8+ CD28- lymphocytes from HIV+ patients.Journal of clinical immunology · 2001Article
- Lack of evidence for the Th2 predominance in patients with chronic hepatitis C.Clinical and experimental immunology · 2001Article
- Cytokine analysis at the single cell level and lymphoproliferative responses to mycobacterial antigens in HIV-1 patients with successful virologic response to potent antiretrovirals.Journal of clinical immunology · 2000Article
- Thalidomide analogue CC-3052 reduces HIV+ neutrophil apoptosis in vitro.Clinical and experimental immunology · 2000Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
We used three-colour cytometry to analyse intracellular cytokine production in activated whole blood cultures derived from patients with HIV-1 infection. We assessed mitogen-induced IL-2, IL-4 and IFN-gamma production from T cells as possible markers of immune dysfunction. The percentages of T cells staining for IL-2 were significantly reduced in stimulated cultures from HIV+ individuals relative to normal controls (P<0.0001); this reduction was observed in both the CD4+ and the CD8+ subsets. IL-2 production was significantly reduced in CD4+ T cells from HIV+ individuals clinically classified as symptomatics compared with HIV+ asymptomatics (P<0.001); in addition, production of IL-2 inversely correlated with viral load (r2=0.832). On the other hand, HIV+ individuals showed significantly more T cells staining positive for IFN-gamma (P<0.0001); subset analysis identified these T cells as CD8+. Increased IFN-gamma production in the CD8+ T cell subset of HIV+ individuals correlated neither with clinical status nor with plasma viral load. IL-4 staining in activated T cells was low (<5%) and no differences were observed between HIV+ and control groups. Three-colour FACS analysis of whole blood provides a sensitive, rapid and relatively easy means to detect cytokine profiles within T cell subpopulations. Only small volumes of blood are required (0.5 ml), since there is no need for cell isolation, making it more practical than ELISA or reverse transcriptase-polymerase chain reaction (RT-PCR) for the analysis of immune function in HIV+ individuals. This technique could therefore play a role in mapping the dynamics and extent of immune recovery in AIDS patients undergoing triple combination therapy.
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