ArticleCell motility and the cytoskeleton1998
Rapid and efficient purification of actin from nonmuscle sources.
Article in Cell motility and the cytoskeleton, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Actin Filament Pointed Ends: Assays for Regulation of Assembly and Disassembly by Tropomodulin and Tropomyosin.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Purification of Human Cytoplasmic Actins FromBio-protocol · 2023Article
- Purification of modified mammalian actin isoforms for in vitro reconstitution assays.European journal of cell biology · 2023Review
- Purification of human β- and γ-actin from budding yeast.Journal of cell science · 2023Article
- Ofloxacin as a Disruptor of Actin Aggresome "Hirano Bodies": A Potential Repurposed Drug for the Treatment of Neurodegenerative Diseases.Frontiers in aging neuroscience · 2020Article
- Enterovirus pathogenesis requires the host methyltransferase SETD3.Nature microbiology · 2019Article
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
- Kinase MPK17 and the Peroxisome Division Factor PMD1 Influence Salt-induced Peroxisome Proliferation.Plant physiology · 2018Article
- Actin and Actin-Binding Proteins.Cold Spring Harbor perspectives in biology · 2016Review
- Developmental but not adult cannabinoid treatments persistently alter axonal and dendritic morphology within brain regions important for zebra finch vocal learning.Brain research · 2014Article
- Novel song-stimulated dendritic spine formation and Arc/Arg3.1 expression in zebra finch auditory telencephalon are disrupted by cannabinoid agonism.Brain research · 2013Article
- Biochemical and cell biological analysis of actin in the nematode Caenorhabditis elegans.Methods (San Diego, Calif.) · 2012Article
- Conformational dynamics of actin: effectors and implications for biological function.Cytoskeleton (Hoboken, N.J.) · 2010Review
- Arginylation regulates intracellular actin polymer level by modulating actin properties and binding of capping and severing proteins.Molecular biology of the cell · 2010Article
- Dual roles of Gln137 of actin revealed by recombinant human cardiac muscle alpha-actin mutants.The Journal of biological chemistry · 2008Article
- Identification and characterization of a Ca2+-dependent actin filament-severing protein from lily pollen.Plant physiology · 2004Article
- Signal-mediated depolymerization of actin in pollen during the self-incompatibility response.The Plant cell · 2002Article
- Vertebrate isoforms of actin capping protein beta have distinct functions In vivo.The Journal of cell biology · 1999Article
- Purification of multiple functional leaf-actin isoforms from Phaseolus vulgaris L.The Biochemical journal · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The need for biochemical quantities of nonmuscle actin has been increased by observations that actin isoform composition of a cell influences the cell's motile and structural properties. In addition, the number of actin binding proteins that exhibit different binding interactions with beta- and gamma-actin compared to alpha-actin from skeletal muscle is growing. We report a procedure designed to purify actin from nonmuscle tissues employing extraction of monomeric actin from tissues with high concentrations of Tris, chromatography on DE-53 cellulose, and affinity chromatography of DNase I-agarose. The preparation is easy to perform and yields quantities of nonmuscle actin sufficient for biochemical and cell biological assays. Actin from bovine erythrocytes and from brains of adult and embryonic chickens was obtained using this method, which can be readily used with other sources of tissue. Coomassie-Blue-stained SDS gels of the purified actin show no contaminants; capping protein, a common contaminant of actin preparations, is absent by immunoblotting. This method for purifying nonmuscle actin will be useful to investigate functional differences in the biology of actin isoforms or their regulating proteins.
Indexed as
Identifiers
9484958PMC2362386W2039495884What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.