ArticlePlant molecular biology1998
Characterisation of transcript initiation sites in ribosome-deficient barley plastids.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 69 citations in OpenAlex.
- Professor Thomas Börner: the pioneer of molecular plant genetics in East Germany turns 80!Molecular genetics and genomics : MGG · 2026Article
- High-throughput discovery of plastid genes causing albino phenotypes in ornamental chimeric plants.Horticulture research · 2023Article
- Plastid Gene Transcription: An Update on Promoters and RNA Polymerases.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Systematic sequencing of chloroplast transcript termini from Arabidopsis thaliana reveals >200 transcription initiation sites and the extensive imprints of RNA-binding proteins and secondary structures.Nucleic acids research · 2019Article
- Cryptochrome 2 extensively regulates transcription of the chloroplast genome in tomato.FEBS open bio · 2017Article
- Mechanism of Dual Targeting of the Phytochrome Signaling Component HEMERA/pTAC12 to Plastids and the Nucleus.Plant physiology · 2017Article
- WSL3, a component of the plastid-encoded plastid RNA polymerase, is essential for early chloroplast development in rice.Plant molecular biology · 2016Article
- Characterization of the psbH precursor RNAs reveals a precise endoribonuclease cleavage site in the psbT/psbH intergenic region that is dependent on psbN gene expression.Plant molecular biology · 2015Article
- The primary transcriptome of barley chloroplasts: numerous noncoding RNAs and the dominating role of the plastid-encoded RNA polymerase.The Plant cell · 2012Article
- Reverse protection assay: a tool to analyze transcriptional rates from individual promoters.Plant methods · 2011Article
- The foundation of extranuclear inheritance: plastid and mitochondrial genetics.Molecular genetics and genomics : MGG · 2010Review
- Intraplastidial trafficking of a phage-type RNA polymerase is mediated by a thylakoid RING-H2 protein.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner.Molecular genetics and genomics : MGG · 2008Article
- Impaired function of the phage-type RNA polymerase RpoTp in transcription of chloroplast genes is compensated by a second phage-type RNA polymerase.Nucleic acids research · 2008Article
- Phage-type RNA polymerase RPOTmp transcribes the rrn operon from the PC promoter at early developmental stages in Arabidopsis.Plant physiology · 2007Article
- Article
- Unique architecture of the plastid ribosomal RNA operon promoter recognized by the multisubunit RNA polymerase in tobacco and other higher plants.The Plant cell · 2003Article
- Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases.The EMBO journal · 2000Article
- Identification of two essential sequence elements in the nonconsensus type II PatpB-290 plastid promoter by using plastid transcription extracts from cultured tobacco BY-2 cells.The Plant cell · 1999Article
- In vitro characterization of the tobacco rpoB promoter reveals a core sequence motif conserved between phage-type plastid and plant mitochondrial promoters.The EMBO journal · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start sites of the genes clpP and rpl23 in ribosome-free plastids of the barley mutant albostrians. No transcript initiation was found at sequences similar to eubacterial promoters. Instead, transcription started near the motif 5'-YRTA-3', which is also conserved in mitochondrial promoters of higher plants. Our data suggest that the nuclear encoded RNA polymerase is active in mature chloroplasts and is the sole polymerase involved in transcription of rpl123.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.