ArticlePlant molecular biology1998
Implication of 5'-flanking sequence elements in expression of a plant tRNA(Leu) gene.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- From water to land: evolution of tRNA gene repertoires in photosynthetic organisms.The EMBO journal · 2026Review
- Multilevel genomic constraints shape nuclear tRNA gene organization in plants.The Plant journal : for cell and molecular biology · 2026Article
- tRNA gene content, structure, and organization in the flowering plant lineage.Frontiers in plant science · 2024Article
- Epigenetic silencing of clustered tRNA genes in Arabidopsis.Nucleic acids research · 2020Article
- Circularly permuted tRNA genes: their expression and implications for their physiological relevance and development.Frontiers in genetics · 2014Review
- Improvement of the pBI121 plant expression vector by leader replacement with a sequence combining a poly(CAA) and a CT motif.Transgenic research · 2007Article
- Visualizing bz1 missense suppression in Zea mays: an assay for monocot tRNA expression and utilization.Plant molecular biology · 2006Article
- Translational nonsense codon suppression as indicator for functional pre-tRNA splicing in transformed Arabidopsis hypocotyl-derived calli.Nucleic acids research · 2003Article
- Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeast.Molecular and cellular biology · 2001Article
- Plant cytosolic tRNAHis possesses an exceptional C54 in the canonical TPsiC loop.Nucleic acids research · 1998Article
- Structure and expression of several bean (Phaseolus vulgaris) nuclear transfer RNA genes: relevance to the process of tRNA import into plant mitochondria.Plant molecular biology · 1998Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A comparison of 5'-flanking sequences from 68 different nuclear plant tRNA genes was analyzed to find consensus sequences. Three conserved features stood out, all of which are present in the tRNA(Leu) gene used in this study: (1) a high proportion of A and T residues upstream of all tRNA genes; (2) a region of low duplex stability about 30-35 bp before the coding sequence, often containing a TATA-box like motif; (3) a CAA triplet in the region of the presumed transcription start. The effect of replacement of the AT-rich upstream sequences with GC-rich sequences or unrelated AT-rich sequences was tested by progressive deletions and by inserting randomly cloned sequences upstream of the tRNA gene. GC-rich 5'-flanking sequences were found to be generally incompatible with high levels of expression. The TATA-box like motifs and the CAA triplet were removed or altered by deletion or directed mutagenesis. Mutation of the CAA triplet significantly decreased expression of the tRNA(Leu) gene, suggesting that this CAA triplet is important for transcription efficiency, but mutation or elimination of the TATA-box like motifs generally had little effect. The presence or absence of each of these features in tRNA genes from other organisms is discussed; there are clear and interesting differences between plant tRNA genes and those of yeast and mammals.
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