ArticleThe EMBO journal1998
Isolation and characterization of Dcp1p, the yeast mRNA decapping enzyme.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
77 citing papers in PubMed, 156 citations in OpenAlex.
- Biochemical and functional characterization of orf virus decapping protein OV71.Virology journal · 2025Article
- Transcriptome-Wide Analysis of the 5' Cap Status of RNA Using 5' Monophosphate-Dependent Exonuclease Digestion and RNA Sequencing.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Eukaryotic mRNA decapping factors: molecular mechanisms and activity.The FEBS journal · 2023Review
- Decoupling of degradation from deadenylation reshapes poly(A) tail length in yeast meiosis.Nature structural & molecular biology · 2021Article
- A Forward Genetic Approach to Mapping aG3 (Bethesda, Md.) · 2020Article
- Article
- LARP1 on TOP of ribosome production.Wiley interdisciplinary reviews. RNA · 2018Review
- Beyond transcription factors: roles of mRNA decay in regulating gene expression in plants.F1000Research · 2018Review
- Interconnections between mRNA degradation and RDR-dependent siRNA production in mRNA turnover in plants.Journal of plant research · 2017Review
- Article
- Maternally recruited DCP1A and DCP2 contribute to messenger RNA degradation during oocyte maturation and genome activation in mouse.Biology of reproduction · 2013Article
- Article
- Dehydration stress activates Arabidopsis MPK6 to signal DCP1 phosphorylation.The EMBO journal · 2012Article
- Down-regulation of Decapping Protein 2 mediates chronic nicotine exposure-induced locomotor hyperactivity in Drosophila.PloS one · 2012Article
- Stm1 modulates translation after 80S formation in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2011Article
- Different nuclease requirements for exosome-mediated degradation of normal and nonstop mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition.RNA (New York, N.Y.) · 2011Article
- Identification and analysis of the interaction between Edc3 and Dcp2 in Saccharomyces cerevisiae.Molecular and cellular biology · 2010Article
- Intrinsic RNA binding by the eukaryotic initiation factor 4F depends on a minimal RNA length but not on the m7G cap.The Journal of biological chemistry · 2009Article
- The Drosophila melanogaster Gene cg4930 Encodes a High Affinity Inhibitor for Endonuclease G.The Journal of biological chemistry · 2009Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major mechanism of mRNA decay occurs by the process of deadenylation, decapping and 5' --> 3' exonucleolytic degradation. Recently, the product of the DCP1 gene has been shown to be required for decapping mRNAs in vivo and co-purifies with decapping activity in vitro. We have purified Dcp1p to homogeneity and shown that it is sufficient for decapping, thereby indicating that Dcp1p is the decapping enzyme. Characterization of Dcp1p activity in vitro indicated that the 7-methyl group of the cap structure contributes to the enzyme's substrate specificity. In addition, Dcp1p was effectively inhibited by uncapped mRNAs, and the enzyme efficiently cleaved substrates that were >/=25 nucleotides in length, with a preference for longer mRNA substrates. These properties suggest that Dcp1p recognizes the mRNA substrate by interactions with both the cap and the RNA moiety. The Dcp1p is also a phosphoprotein, suggesting its activity may be regulated by post-transcriptional modification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.