ArticleThe EMBO journal1998
An embryonic demethylation mechanism involving binding of transcription factors to replicating DNA.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 101 citations in OpenAlex.
- DNA methylation and DNA methyltransferases.Epigenetics & chromatin · 2017Review
- Notes on the role of dynamic DNA methylation in mammalian development.Proceedings of the National Academy of Sciences of the United States of America · 2015Review
- Oct4/Sox2 binding sites contribute to maintaining hypomethylation of the maternal igf2/h19 imprinting control region.PloS one · 2013Article
- The p16-specific reactivation and inhibition of cell migration through demethylation of CpG islands by engineered transcription factors.Human gene therapy · 2012Article
- Characterization of CpG island DNA methylation of impairment-related genes in a rat model of cognitive aging.Epigenetics · 2012Article
- Widespread plasticity in CTCF occupancy linked to DNA methylation.Genome research · 2012Article
- Breast carcinoma cells re-express E-cadherin during mesenchymal to epithelial reverting transition.Molecular cancer · 2010Article
- Chromatin and sequence features that define the fine and gross structure of genomic methylation patterns.Genome research · 2010Article
- Article
- Preferential epigenetic suppression of the autonomous MusD over the nonautonomous ETn mouse retrotransposons.Molecular and cellular biology · 2009Article
- Evidence for a bigenic chromatin subdomain in regulation of the fetal-to-adult hemoglobin switch.Molecular and cellular biology · 2009Article
- Mediators of reprogramming: transcription factors and transitions through mitosis.Nature reviews. Molecular cell biology · 2008Review
- Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics.Nucleic acids research · 2008Article
- Allele-specific demethylation at an imprinted mammalian promoter.Nucleic acids research · 2007Article
- Active cytosine demethylation triggered by a nuclear receptor involves DNA strand breaks.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Towards understanding the epigenetics of transcription by chromatin structure and the nuclear matrix.Gene therapy & molecular biology · 2005Article
- Activity of metal-responsive transcription factor 1 by toxic heavy metals and H2O2 in vitro is modulated by metallothionein.Molecular and cellular biology · 2003Article
- The SDF1-G801A polymorphism is not associated with SDF1 gene expression in Epstein-Barr virus-transformed lymphoblastoid cells.Genes and immunity · 2003Article
- Inducible DNA demethylation mediated by the maize Suppressor-mutator transposon-encoded TnpA protein.The Plant cell · 2002Article
- The barrier function of an insulator couples high histone acetylation levels with specific protection of promoter DNA from methylation.Genes & development · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vertebrates, transcriptionally active promoters are undermethylated. Since the transcription factor Sp1, and more recently NF-kappaB, have been implicated in the demethylation process, we examined the effect of transcription factors on demethylation by injecting in vitro methylated plasmid DNA into Xenopus fertilized eggs. We found that various transactivation domains, including a strong acidic activation domain from the viral protein VP16, can enhance demethylation of a promoter region when fused to a DNA binding domain which recognizes the promoter. Furthermore, demethylation occurs only after the midblastula transition, when the general transcription machinery of the host embryo becomes available. Nevertheless, transcription factor binding need not be followed by actual transcription, since demethylation is not blocked by alpha-amanitin treatment. Finally, replication of the target DNA is a prerequisite for efficient demethylation since only plasmids that carry the bovine papilloma virus sequences which support plasmid replication after the midblastula transition are demethylated. No demethylation is detectable in the oocyte system where DNA is not replicated. These results suggest that, in the Xenopus embryo, promoters for which transcription factors are available are demethylated by a replication-dependent, possibly passive mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.