ArticleThe EMBO journal1998
Ca2+-induced exocytosis in individual human neutrophils: high- and low-affinity granule populations and submaximal responses.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- NCX1 reverse mode promotes calcium-dependent Neutrophil Extracellular Trap formation and lung damage in chronic obstructive pulmonary disease.Nature communications · 2026Article
- Regulation of Neutrophil Functions by Hv1/VSOP Voltage-Gated Proton Channels.International journal of molecular sciences · 2021Review
- N-acetyl-L-cysteine and cysteine increase intracellular calcium concentration in human neutrophils.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2016Article
- The role of STIM and ORAI proteins in phagocytic immune cells.American journal of physiology. Cell physiology · 2016Review
- E-selectin ligands as mechanosensitive receptors on neutrophils in health and disease.Annals of biomedical engineering · 2012Review
- Cdk5 mediates vimentin Ser56 phosphorylation during GTP-induced secretion by neutrophils.Journal of cellular physiology · 2012Article
- Inhibitors of TRP channels reveal stimulus-dependent differential activation of Ca2+ influx pathways in human neutrophil granulocytes.Naunyn-Schmiedeberg's archives of pharmacology · 2009Article
- Calcium flux in neutrophils synchronizes beta2 integrin adhesive and signaling events that guide inflammatory recruitment.Annals of biomedical engineering · 2008Article
- Neutrophil secretion induced by an intracellular Ca2+ rise and followed by whole-cell patch-clamp recordings occurs without any selective mobilization of different granule populations.Journal of biomedicine & biotechnology · 2006Article
- Rapid biogenesis and sensitization of secretory lysosomes in NK cells mediated by target-cell recognition.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The two neutrophil members of the formylpeptide receptor family activate the NADPH-oxidase through signals that differ in sensitivity to a gelsolin derived phosphoinositide-binding peptide.BMC cell biology · 2004Article
- Holding back neutrophil aggression; the oxidase has potential.Clinical and experimental immunology · 2003Review
- A highly Ca2+-sensitive pool of vesicles is regulated by protein kinase C in adrenal chromaffin cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Differential exocytosis from human endothelial cells evoked by high intracellular Ca(2+) concentration.The Journal of physiology · 2002Article
- Modeling excess retrieval in rat melanotroph membrane capacitance records.Biophysical journal · 2002Article
- A kinetic analysis of calcium-triggered exocytosis.The Journal of general physiology · 2001Article
- Microdomains of high calcium are not required for exocytosis in RBL-2H3 mucosal mast cells.The Journal of cell biology · 2001Article
- Yersinia pseudotuberculosis-induced calcium signaling in neutrophils is blocked by the virulence effector YopH.Infection and immunity · 1999Article
- Exocytosis and endocytosisThe Plant cell · 1999Article
- Membrane capacitance changes induced by thrombin and calcium in single endothelial cells cultured from human umbilical vein.The Journal of physiology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated Ca2+-induced exocytosis from human neutrophils using the whole cell patch-clamp capacitance technique. Microperfusion of Ca2+ buffer solutions (<30 nM to 5 mM free Ca2+) through the patch-clamp pipette revealed a biphasic activation of exocytosis by Ca2+. The first phase was characterized by high affinity (1.5-5 microM) and low apparent cooperativity (<=2) for Ca2+, and the second phase by low affinity (approximately 100 microM) and high cooperativity (>6). Only the second phase was accompanied by loss of myeloperoxidase, suggesting that the low-affinity exocytosis reflected release of peroxidase-positive (primary) granules, while the high-affinity exocytosis reflected release of peroxidase-negative (secondary and tertiary) granules. At submaximal Ca2+ concentrations, only a fraction of a given granule population was released. This submaximal release cannot simply be explained by Ca2+ modulation of the rate of exocytosis, and it suggests that the secretory response of individual cells is adjusted to the strength of the stimulus. The Ca2+ dependence of the high- and low-affinity phases of neutrophil exocytosis bears a resemblance to endocrine and neuronal exocytosis, respectively. The occurrence of such high- and low-affinity exocytosis in the same cell is novel, and suggests that the Ca2+ sensitivity of secretion is granule-, rather than cell-specific.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.