ArticleThe Journal of physiology1998
The channel opening rate of adult- and fetal-type mouse muscle nicotinic receptors activated by acetylcholine.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Article
- Full and partial agonists evoke distinct structural changes in opening the muscle acetylcholine receptor channel.The Journal of general physiology · 2018Article
- Improved resolution of single channel dwell times reveals mechanisms of binding, priming, and gating in muscle AChR.The Journal of general physiology · 2016Article
- Muscarinic inhibition of nicotinic transmission in rat sympathetic neurons and adrenal chromaffin cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Article
- Hydrophobic Gating of Ion Permeation in Magnesium Channel CorA.PLoS computational biology · 2015Article
- Mechanism of activation of the prokaryotic channel ELIC by propylamine: a single-channel study.The Journal of general physiology · 2015Article
- An outline of desensitization in pentameric ligand-gated ion channel receptors.Cellular and molecular life sciences : CMLS · 2013Review
- Anesthetic sites and allosteric mechanisms of action on Cys-loop ligand-gated ion channels.Canadian journal of anaesthesia = Journal canadien d'anesthesie · 2011Review
- Conformational changes in the nicotinic acetylcholine receptor during gating and desensitization.Biochemistry · 2010Article
- Agonist and blocking actions of choline and tetramethylammonium on human muscle acetylcholine receptors.The Journal of physiology · 2009Article
- Detection and trapping of intermediate states priming nicotinic receptor channel opening.Nature · 2009Article
- Time-resolved photolabeling of the nicotinic acetylcholine receptor by [3H]azietomidate, an open-state inhibitor.Molecular pharmacology · 2009Article
- Agonist-dependent single channel current and gating in alpha4beta2delta and alpha1beta2gamma2S GABAA receptors.The Journal of general physiology · 2008Article
- Differential effects of serotonin and dopamine on human 5-HT3A receptor kinetics: interpretation within an allosteric kinetic model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- The pre-M1 segment of the alpha1 subunit is a transduction element in the activation of the GABAA receptor.The Journal of physiology · 2006Article
- Plasticity of acetylcholine receptor gating motions via rate-energy relationships.Biophysical journal · 2005Article
- Role of aspartate 298 in mouse 5-HT3A receptor gating and modulation by extracellular Ca2+.The Journal of physiology · 2005Article
- Activation of heteroliganded mouse muscle nicotinic receptors.The Journal of physiology · 2005Article
- Ligand-induced conformational change in the alpha7 nicotinic receptor ligand binding domain.Biophysical journal · 2005Article
- Gating dynamics of the acetylcholine receptor extracellular domain.The Journal of general physiology · 2004Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
1. In this paper, we examine acetylcholine (ACh)-induced currents in quail fibroblast cell lines expressing either the fetal (Q-F18) or the adult (Q-A33) complement of nicotinic acetylcholine receptor subunits derived from mouse skeletal muscle. Pulses of ACh were applied to outside-out patches of cell membrane by means of a fast perfusion system at concentrations from 100 nM to 10 mM. We obtained current records with intracellular potentials of -60 and +40 mV. The goal of this study was to estimate the channel opening rate. 2. By fitting sums of exponentials to averaged responses, we estimated the rate of development of the current on the application of acetylcholine. The rate constant of the predominant exponential component (the on-rate) ranges over 3 orders of magnitude, from around 100 s-1 (fetal) at low concentrations ACh to over 100,000 s-1 (fetal and adult) at the highest concentrations. 3. We establish that our measurement of the on-rate is not limited by technical constraints, and can therefore be related to the rate constants of a kinetic scheme. Our observations are consistent with a model having a rate-limiting channel opening step with a forward rate constant (beta) of 80,000 s-1 on average for adult receptors and 60,000 s-1 for fetal receptors, and a minimum opening to closing ratio (beta/alpha) of around 33 (adult) or 50 (fetal). The channel opening rate, beta, varies from around 30,000 s-1 to well over 100,000 s-1 for different patches. The large variation cannot all be ascribed to errors of measurement, but indicates patch to patch variation.
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