ArticleThe Biochemical journal1998
Pseudo-enzymatic S-acylation of a myristoylated yes protein tyrosine kinase peptide in vitro may reflect non-enzymatic S-acylation in vivo.
Article in The Biochemical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Single-residue posttranslational modification sites at the N-terminus, C-terminus or in-between: To be or not to be exposed for enzyme access.Proteomics · 2015Article
- Regulation of Ras localization and cell transformation by evolutionarily conserved palmitoyltransferases.Molecular and cellular biology · 2014Article
- N-terminal palmitoylation is required for Toxoplasma gondii HSP20 inner membrane complex localization.Biochimica et biophysica acta · 2013Article
- Post-translational modification and regulation of actin.Current opinion in cell biology · 2013Review
- Roles of cysteine residues in the inhibition of human glutamate dehydrogenase by palmitoyl-CoA.BMB reports · 2012Article
- A palmitoylation switch mechanism regulates Rac1 function and membrane organization.The EMBO journal · 2012Article
- Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.Molecular & cellular proteomics : MCP · 2011Article
- Characterization of the self-palmitoylation activity of the transport protein particle component Bet3.Cellular and molecular life sciences : CMLS · 2010Article
- Palmitoylation of cytoskeleton associated protein 4 by DHHC2 regulates antiproliferative factor-mediated signaling.Molecular biology of the cell · 2009Article
- Identification of palmitoylated mitochondrial proteins using a bio-orthogonal azido-palmitate analogue.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- Palmitylation of cone opsins.Vision research · 2006Article
- Unique self-palmitoylation activity of the transport protein particle component Bet3: a mechanism required for protein stability.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues.The Biochemical journal · 2004Article
- On the mechanism of protein palmitoylation.EMBO reports · 2004Review
- The SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusion.The EMBO journal · 2004Article
- Characterization of human palmitoyl-acyl transferase activity using peptides that mimic distinct palmitoylation motifs.The Biochemical journal · 2003Article
- Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion.The EMBO journal · 2001Article
- Regulation of G proteins by covalent modification.Oncogene · 2001Review
- Autopalmitoylation of tubulin.Protein science : a publication of the Protein Society · 2000Article
- Palmitoylation of the 25-kDa synaptosomal protein (SNAP-25) in vitro occurs in the absence of an enzyme, but is stimulated by binding to syntaxin.The Biochemical journal · 2000Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Covalent attachment of a variety of lipid groups to proteins is now recognized as a major group of post-translational modifications. S-acylation of proteins at cysteine residues is the only modification considered dynamic and thus has the potential for regulating protein function and/or localization. The activities that catalyse reversible S-acylation have not been well characterized and it is not clear whether both the acylation and the deacylation steps are regulated, since in principle it would be sufficient to control only one of them. Both apparently enzymatic and non-enzymatic S-acylation of proteins have previously been reported. Here we show that a synthetic myristoylated c-Yes protein tyrosine kinase undecapeptide undergoes spontaneous S-acylation in vitro when using a long chain acyl-CoA as acyl donor in the absence of any protein. The S-acylation was dependent on myristoylation of the substrate, the length of the incubation period, temperature and substrate concentration. When COS cell fractions were added to the S-acylation reaction no additional peptide:S-acyltransferase activity was detected. These results are consistent with the possibility that membrane-associated proteins may undergo S-acylation in vivo by non-enzymatic transfer of acyl groups from acyl-CoA. In this case, the S-acylation-deacylation process could be controlled by a regulated depalmitoylation mechanism.
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