ArticleGenetics1998
A mutation in Paramecium tetraurelia reveals functional and structural features of developmentally excised DNA elements.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Massive colonization of protein-coding exons by selfish genetic elements in Paramecium germline genomes.PLoS biology · 2021Article
- Programmed genome rearrangements in ciliates.Cellular and molecular life sciences : CMLS · 2020Review
- Cis-acting signals modulate the efficiency of programmed DNA elimination in Paramecium tetraurelia.Nucleic acids research · 2015Article
- Genome-wide analysis of genetic and epigenetic control of programmed DNA deletion.Nucleic acids research · 2014Article
- Pdsg1 and Pdsg2, novel proteins involved in developmental genome remodelling in Paramecium.PloS one · 2014Article
- The Paramecium germline genome provides a niche for intragenic parasitic DNA: evolutionary dynamics of internal eliminated sequences.PLoS genetics · 2012Article
- Transposon Invasion of the Paramecium Germline Genome Countered by a Domesticated PiggyBac Transposase and the NHEJ Pathway.International journal of evolutionary biology · 2012Article
- Developmentally programmed DNA splicing in Paramecium reveals short-distance crosstalk between DNA cleavage sites.Nucleic acids research · 2008Article
- Analysis of sequence variability in the macronuclear DNA of Paramecium tetraurelia: a somatic view of the germline.Genome research · 2008Article
- Developmentally regulated chromosome fragmentation linked to imprecise elimination of repeated sequences in paramecia.Eukaryotic cell · 2003Article
- Control of DNA excision efficiency in Paramecium.Nucleic acids research · 2001Article
- Developmentally regulated excision of a 28-base-pair sequence from the Paramecium genome requires flanking DNA.Molecular and cellular biology · 2000Article
- Article
- Timing of developmentally programmed excision and circularization of Paramecium internal eliminated sequences.Molecular and cellular biology · 2000Article
- Article
- Homology-dependent maternal inhibition of developmental excision of internal eliminated sequences in Paramecium tetraurelia.Molecular and cellular biology · 1998Article
- Developmental timing of programmed DNA elimination inGenome researchArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The excision of internal eliminated sequences (IESs) from the germline micronuclear DNA occurs during the differentiation of a new macronuclear genome in ciliated protozoa. In Paramecium, IESs are generally short (28-882 bp), AT rich DNA elements that show few conserved sequence features with the exception of an inverted-terminal-repeat consensus sequence that has similarity to the ends of mariner/Tcl transposons (KLOBUTCHER and HERRICK 1995). We have isolated and analyzed a mutant cell line that cannot excise a 370-bp IESs (IES2591) from the coding region of the 51A variable surface protein gene. A single micronuclear C to T transition within the consensus sequence prevents excision. The inability to excise IES259 I has revealed a 28-bp IES inside the larger IES, suggesting that reiterative integration of these elements can occur. Together, the consensus sequence mutation and the evidence for reiterative integration support the theory that Paramecium IESs evolved from transposable elements. Unlike a previously studied Paramecium IES, the presence of this IES in the macronucleus does not completely inhibit excision of its Mild-type micronuclear copy through multiple sexual generations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.