ArticleGenetics1998
Isolation of a Schizosaccharomyces pombe rad21ts mutant that is aberrant in chromosome segregation, microtubule function, DNA repair and sensitive to hydroxyurea: possible involvement of Rad21 in ubiquitin-mediated proteolysis.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 75 citations in OpenAlex.
- Humanization of theGenes · 2026Article
- Site-to-site mutational dissection of fission yeast cohesin reveals its dynamics.G3 (Bethesda, Md.) · 2025Article
- Cohesin mutations in acute myeloid leukemia.Leukemia · 2024Review
- Review
- Kinetochore-mediated outward force promotes spindle pole separation in fission yeast.Molecular biology of the cell · 2019Article
- The Functional 3D Organization of Unicellular Genomes.Scientific reports · 2019Article
- Article
- Suppressor screening reveals common kleisin-hinge interaction in condensin and cohesin, but different modes of regulation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Isolation of Fission Yeast Condensin Temperature-Sensitive Mutants with Single Amino Acid Substitutions Targeted to Hinge Domain.G3 (Bethesda, Md.) · 2019Article
- Mutations that prevent methylation of cohesin render sensitivity to DNA damage inJournal of cell science · 2018Article
- Suppressor mutation analysis combined with 3D modeling explains cohesin's capacity to hold and release DNA.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Review
- Transcription facilitates sister chromatid cohesion on chromosomal arms.Nucleic acids research · 2016Article
- Nse1-dependent recruitment of Smc5/6 to lesion-containing loci contributes to the repair defects of mutant complexes.Molecular biology of the cell · 2011Article
- Psm3 acetylation on conserved lysine residues is dispensable for viability in fission yeast but contributes to Eso1-mediated sister chromatid cohesion by antagonizing Wpl1.Molecular and cellular biology · 2011Article
- Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation.Molecular and cellular biology · 2011Article
- Checkpoint-dependent and -independent roles of Swi3 in replication fork recovery and sister chromatid cohesion in fission yeast.PloS one · 2010Article
- Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast.The Journal of cell biology · 2009Article
- The Drosophila cohesin subunit Rad21 is a trithorax group (trxG) protein.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Schizosaccharomyces pombe Bub3 is dispensable for mitotic arrest following perturbed spindle formation.Genetics · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fission yeast DNA repair gene rad21+ is essential for cell growth. To investigate the function essential for cell proliferation, we have isolated a temperature-sensitive mutant of the rad21+ gene. The mutant, rad21-K1, showed abnormal mitosis at the nonpermissive temperature. Some cells contained abnormal nuclear structures, such as condensed chromosomes with short spindles, or chromosomes stretched or unequally separated by elongating spindles. Other cells exhibited the displaced nucleus or a cut-like phenotype. Similar abnormalities were observed when the Rad21 protein was depleted from cells. We therefore concluded that Rad21 is essential for proper segregation of chromosomes. Moreover, the rad21-K1 mutant is sensitive not only to UV and gamma-ray irradiation but to thiabendazole and hydroxyurea, indicating that Rad21 plays important roles in microtubule function, DNA repair, and S phase function. The relation to the microtubule function was further confirmed by the fact that rad21+ genetically interacts with tubulin genes, nda2+ and nda3+. Finally, the growth of the rad21-K1 mutant was inhibited at the permissive temperature by introduction of another mutation in the cut9+ gene, coding for a component of the 20S cyclosome/anaphase promoting complex, which is involved in ubiquitin-mediated proteolysis. The results suggest that these diverse functions of Rad21 may be facilitated through ubiquitin-mediated proteolysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.