ArticleJournal of bacteriology1998
Identification of AlcR, an AraC-type regulator of alcaligin siderophore synthesis in Bordetella bronchiseptica and Bordetella pertussis.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 45 citations in OpenAlex.
- Prevalence and variability of siderophore production in theMicrobiology spectrum · 2024Article
- Pathogenicity and virulence ofVirulence · 2021Review
- Article
- Iron homeostasis in theAdvances in botanical research · 2013Article
- The iron-repressed, AraC-like regulator MpeR activates expression of fetA in Neisseria gonorrhoeae.Infection and immunity · 2011Article
- Temporal signaling and differential expression of Bordetella iron transport systems: the role of ferrimones and positive regulators.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2009Review
- Differential expression of Bordetella pertussis iron transport system genes during infection.Molecular microbiology · 2008Article
- The AraC-like transcriptional regulator DhbR is required for maximum expression of the 2,3-dihydroxybenzoic acid biosynthesis genes in Brucella abortus 2308 in response to iron deprivation.Journal of bacteriology · 2008Article
- Impact of alcaligin siderophore utilization on in vivo growth of Bordetella pertussis.Infection and immunity · 2007Article
- Expression of hurP, a gene encoding a prospective site 2 protease, is essential for heme-dependent induction of bhuR in Bordetella bronchiseptica.Journal of bacteriology · 2007Article
- Computational reconstruction of iron- and manganese-responsive transcriptional networks in alpha-proteobacteria.PLoS computational biology · 2006Article
- Characterization of a highly conserved island in the otherwise divergent Bordetella holmesii and Bordetella pertussis genomes.Journal of bacteriology · 2006Article
- Bordetella AlcS transporter functions in alcaligin siderophore export and is central to inducer sensing in positive regulation of alcaligin system gene expression.Journal of bacteriology · 2005Article
- Regulation of mtrF expression in Neisseria gonorrhoeae and its role in high-level antimicrobial resistance.Journal of bacteriology · 2005Article
- Integration of environmental signals controls expression of Bordetella heme utilization genes.Journal of bacteriology · 2004Article
- Heme-responsive transcriptional activation of Bordetella bhu genes.Journal of bacteriology · 2003Article
- Alcaligin siderophore production by Bordetella bronchiseptica strain RB50 is not repressed by the BvgAS virulence control system.Journal of bacteriology · 2002Article
- Genetic complementation of an outer membrane cytochrome omcB mutant of Shewanella putrefaciens MR-1 requires omcB plus downstream DNA.Applied and environmental microbiology · 2002Article
- Bordetella interspecies allelic variation in AlcR inducer requirements: identification of a critical determinant of AlcR inducer responsiveness and construction of an alcR(Con) mutant allele.Journal of bacteriology · 2002Article
- Identification and characterization of pvuA, a gene encoding the ferric vibrioferrin receptor protein in Vibrio parahaemolyticus.Journal of bacteriology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A Fur titration assay was used to isolate DNA fragments bearing putative Fur binding sites (FBS) from a partial Bordetella bronchiseptica genomic DNA library. A recombinant plasmid bearing a 3.5-kb DNA insert was further studied. Successive deletions in the cloned fragment enabled us to map a putative FBS at about 2 kb from one end. Sequence analysis revealed the presence of an FBS upstream from a new gene encoding an AraC-type transcriptional regulator. The deduced protein displays similarity to PchR, an activator of pyochelin siderophore and ferripyochelin receptor synthesis in Pseudomonas aeruginosa. Homologous genes in Bordetella pertussis and Bordetella parapertussis were PCR amplified, and sequence comparisons indicated a very high conservation in the three species. The B. pertussis and B. bronchiseptica chromosomal genes were inactivated by allelic exchange. Under low-iron growth conditions, the mutants did not secrete the alcaligin siderophore and lacked AlcC, an alcaligin biosynthetic enzyme. Alcaligin production was restored after transformation with a plasmid bearing the wild-type gene. On the basis of its role in regulation of alcaligin biosynthesis, the new gene was designated alcR. Additional sequence determination showed that alcR is located about 2 kb downstream from the alcABC operon and is transcribed in the same orientation. Two tightly linked open reading frames, alcD and alcE, were identified between alcC and alcR. AlcE is a putative iron-sulfur protein; AlcD shows no homology with the proteins in the database. The production of major virulence factors and colonization in the mouse respiratory infection model are AlcR independent.
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