ArticleThe Histochemical journal1997
In situ detection of spontaneous superoxide anion and singlet oxygen production by mitochondria in rat liver and small intestine.
Article in The Histochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 72 citations in OpenAlex.
- Article
- Radical pairs may explain reactive oxygen species-mediated effects of hypomagnetic field on neurogenesis.PLoS computational biology · 2022Article
- Radical pairs may play a role in microtubule reorganization.Scientific reports · 2022Article
- Radical pairs can explain magnetic field and lithium effects on the circadian clock.Scientific reports · 2022Article
- Entangled radicals may explain lithium effects on hyperactivity.Scientific reports · 2021Article
- Quantitative kinetics of intracellular singlet oxygen generation using a fluorescence probe.Scientific reports · 2020Article
- The synergistic effect between hydrogen peroxide and nitrite, two long-lived molecular species from cold atmospheric plasma, triggers tumor cells to induce their own cell death.Redox biology · 2019Article
- The Contribution of Singlet Oxygen to Insulin Resistance.Oxidative medicine and cellular longevity · 2017Review
- Endogenous Generation of Singlet Oxygen and Ozone in Human and Animal Tissues: Mechanisms, Biological Significance, and Influence of Dietary Components.Oxidative medicine and cellular longevity · 2016Review
- In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet.Oxidative medicine and cellular longevity · 2016Article
- Lipopolysaccharide-induced epithelial monoamine oxidase mediates alveolar bone loss in a rat chronic wound model.The American journal of pathology · 2009Article
- In situ assessment of oxidant and nitrogenic stress in bleomycin pulmonary fibrosis.Histochemistry and cell biology · 2006Article
- Benzodiazepine-induced superoxide signals B cell apoptosis: mechanistic insight and potential therapeutic utility.The Journal of clinical investigation · 2002Article
- Morphologic assessment of oxidative damage: A review.Journal of the American Aging Association · 2000Article
- In situ detection of constitutive superoxide anion production in granules of mast cells.The Histochemical journal · 1997Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, the endogenous formation of reactive oxygen species was localized in rat liver and small intestine. The 3,3'-diaminobenzidine (DAB)-Mn2+ technique in which cobalt ions were included in the incubation medium was applied to unfixed cryostat sections of intact tissues. Addition of manganese ions to the DAB-Co(2+)-containing medium greatly increased the amounts of final reaction product formed compared with incubations with only DAB and cobalt ions. In liver, a blue final reaction product was deposited, particularly in hepatocytes surrounding portal tracts. In the small intestine, the DAB-cobalt complex was mainly found at the basal side of enterocytes. Goblet cells remained unstained. Electron microscopical images revealed that an electron-dense reaction product was exclusively present at both inner and outer membranes and at the intermembrane space in mitochondria of liver parenchymal cells and duodenal enterocytes. It was shown that the spontaneous formation of final reaction product was enzymatic and dependent on the presence of oxygen in the medium. Sulphide decreased the reaction, which may indicate that cytochrome c oxidase was partially involved. Benzoquinone and histidine, which are scavengers of superoxide anions and singlet oxygen respectively, reduced the amount of final reaction product considerably. Furthermore, the formation of final reaction product was sensitive to specific inhibitors of NADH:coenzyme Q reductase and aldehyde oxidase, indicating that these enzymes were at least partly responsible for the generation of superoxide anions and singlet oxygen and for the formation of the DAB-cobalt complex.
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