ArticleJournal of clinical microbiology1998
Development and evaluation of a PCR-based assay for detection of Haemobartonella felis in cats and differentiation of H. felis from related bacteria by restriction fragment length polymorphism analysis.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 104 citations in OpenAlex.
- Infection rate of Eperythrozoon spp. in Chinese population: a systematic review and meta-analysis since the first Chinese case reported in 1991.BMC infectious diseases · 2012Pooled it
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- A nosocomial Pseudomonas aeruginosa ST3495 isolated from a wild Burmese python (Python bivittatus) with suppurative pneumonia and bacteremia in Hainan, China.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Mycoplasmas as Host Pantropic and Specific Pathogens: Clinical Implications, Gene Transfer, Virulence Factors, and Future Perspectives.Frontiers in cellular and infection microbiology · 2022Review
- Antigenicity and Safety Evaluation ofFoods (Basel, Switzerland) · 2021Article
- Prevalence of hemoplasmas and Bartonella species in client-owned cats in Beijing and Shanghai, China.The Journal of veterinary medical science · 2021Article
- Review of clinical aspects, epidemiology and diagnosis of haemotropic Mycoplasma ovis in small ruminants: current status and future perspectives in tropics focusing on Malaysia.Tropical animal health and production · 2020Review
- First known case ofThe Journal of international medical research · 2020Article
- Prevalence and risk factor analysis of feline haemoplasma infection in New Zealand domestic cats using a real-time PCR assay.Journal of feline medicine and surgery · 2013Article
- Genome of Mycoplasma haemofelis, unraveling its strategies for survival and persistence.Veterinary research · 2011Article
- Identification, bioinformatics analyses, and expression of immunoreactive antigens of Mycoplasma haemofelis.Clinical and vaccine immunology : CVI · 2011Article
- First morphological characterization of 'Candidatus Mycoplasma turicensis' using electron microscopy.Veterinary microbiology · 2011Article
- The detection of "Candidatus Mycoplasma haemobos" in cattle and buffalo in China.Tropical animal health and production · 2010Article
- Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil).Parasitology research · 2010Article
- Phylogenetic analysis of Mycoplasma suis isolates based on 16S rRNA gene sequence in China.Veterinary research communications · 2009Article
- Development and application of a universal Hemoplasma screening assay based on the SYBR green PCR principle.Journal of clinical microbiology · 2009Article
- The occurrence of the feline "Candidatus Mycoplasma haemominutum" in dog in China confirmed by sequence-based analysis of ribosomal DNA.Tropical animal health and production · 2009Article
- Use of combined conventional and real-time PCR to determine the epidemiology of feline haemoplasma infections in northern Italy.Journal of feline medicine and surgery · 2009Article
- Hemoplasma infection in HIV-positive patient, Brazil.Emerging infectious diseases · 2008Article
- Co-infection with Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in three cats from Brazil.Journal of feline medicine and surgery · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 16S rRNA gene of Haemobartonella felis was amplified by using universal eubacterial primers and was subsequently cloned and sequenced. Based on this sequence data, we designed a set of H. felis-specific primers. These primers selectively amplified a 1,316-bp DNA fragment of the 16S rRNA gene of H. felis from each of four experimentally infected cats at peak parasitemia. No PCR product was amplified from purified DNA of Eperythrozoon suis, Mycoplasma genitalium, and Bartonella bacilliformis. Blood from the experimental cats prior to infection was negative for PCR products and was greatly diminished or absent 1 month after doxycycline treatment. The overall sequence identity of this fragment varied by less than 1.0% among experimentally infected cats. By taking into consideration the secondary structure of the 16S rRNA molecule, we were able to further verify the alignment of nucleotides and quality of our sequence data. In this PCR assay, the minimum detectable number of H. felis organisms was determined to be between 50 and 704. The potential usefulness of restriction enzymes DdeI and MnlI for distinguishing H. felis from closely related bacteria was examined. This is the first report of the utility of PCR-facilitated diagnosis and discrimination of H. felis infection in cats.
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