ArticleJournal of clinical microbiology1998
Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
- Serotypes, MIC-Based Antimicrobial Susceptibility, and Genotypic Diversity ofMicroorganisms · 2026Article
- Causes of Sow Mortality and Risks to Post-Mortem Findings in a Brazilian Intensive Swine Production System.Animals : an open access journal from MDPI · 2022Article
- Novel DNA Markers for Identification of Actinobacillus pleuropneumoniae.Microbiology spectrum · 2022Article
- Complete genome forMicrobial genomics · 2021Article
- Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method.Journal of veterinary science · 2017Article
- The RNA chaperone Hfq promotes fitness of Actinobacillus pleuropneumoniae during porcine pleuropneumonia.Infection and immunity · 2013Article
- Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: A new insight into an old approach.Genetics and molecular biology · 2013Article
- Multiplex PCR assay for detection of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis in lungs of pigs from a slaughterhouse.Folia microbiologica · 2010Article
- DNA microarray-based identification and typing of Actinobacillus pleuropneumoniae.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2009Article
- Genetic diversity of Actinobacillus pleuropneumoniae assessed by amplified fragment length polymorphism analysis.Journal of clinical microbiology · 2007Article
- Detection and identification of Actinobacillus pleuropneumoniae serotypes 1, 2, and 8 by multiplex PCR.Journal of clinical microbiology · 2004Article
- Evaluation and field validation of PCR tests for detection of Actinobacillus pleuropneumoniae in subclinically infected pigs.Journal of clinical microbiology · 2003Article
- Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6.Journal of clinical microbiology · 2003Article
- Evaluation of 5' nuclease assay for detection of Actinobacillus pleuropneumoniae.Journal of clinical microbiology · 2001Article
- Two Actinobacillus pleuropneumoniae serotype 8 reference strains in circulation.Journal of clinical microbiology · 2000Article
- Genomic relatedness among Actinobacillus pleuropneumoniae field strains of sterotypes 1 and 5 isolated from healthy and diseased pigs.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 1999Article
- Molecular cloning and sequencing of the aroA gene from Actinobacillus pleuropneumoniae and its use in a PCR assay for rapid identification.Journal of clinical microbiology · 1999Article
- Detection and identification of Actinobacillus pleuropneumoniae serotype 5 by multiplex PCR.Journal of clinical microbiology · 1998Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene (omlA) coding for an outer membrane protein of Actinobacillus pleuropneumoniae serotypes 1 and 5 has been described earlier and has formed the basis for development of a specific PCR assay. The corresponding regions of all 12 A. pleuropneumoniae reference strains of biovar 1 were sequenced. Alignment of the sequences revealed conserved terminal and variable middle regions, which divided the reference strains into four distinct groups. Primers were selected from the conserved 5' and 3' termini of the gene. A 950-bp amplicon was obtained from each of 102 tested field isolates of A. pleuropneumoniae obtained from lungs. Their identity was verified by sequencing approximately 500 bp of the amplification product from 50 of the A. pleuropneumoniae isolates, which all showed the expected DNA sequence characteristic of the serotype. To test the specificity of the reaction, 23 other bacterial species related to A. pleuropneumoniae or isolated from pigs were assayed. They were all found negative in the PCR, as were tonsil cultures from 50 pigs of an A. pleuropneumoniae-negative herd. The sensitivity assessed by agarose gel analysis of the PCR product was 10(2) CFU/PCR test tube. The specificity and sensitivity of this PCR compared to those of culture suggest the use of this PCR for routine identification of A. pleuropneumoniae.
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