ArticleProceedings of the National Academy of Sciences of the United States of America1998
Identification and characterization of a cell membrane nucleic acid channel.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 130 citations in OpenAlex.
- Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges.International journal of molecular sciences · 2023Review
- Targeting Ephrin Receptor Tyrosine Kinase A2 with a Selective Aptamer for Glioblastoma Stem Cells.Molecular therapy. Nucleic acids · 2020Article
- Phosphorothioate modified oligonucleotide-protein interactions.Nucleic acids research · 2020Article
- Translocation through a narrow pore under a pulling force.Scientific reports · 2019Article
- Unraveling Prion Protein Interactions with Aptamers and Other PrP-Binding Nucleic Acids.International journal of molecular sciences · 2017Review
- Protein Kinase C-α is a Critical Protein for Antisense Oligonucleotide-mediated Silencing in Mammalian Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Properties of internalization factors contributing to the uptake of extracellular DNA into tumor-initiating stem cells of mouse Krebs-2 cell line.Stem cell research & therapy · 2016Article
- Cellular uptake and intracellular trafficking of oligonucleotides.Advanced drug delivery reviews · 2015Review
- Engineering protocells: prospects for self-assembly and nanoscale production-lines.Life (Basel, Switzerland) · 2015Review
- Comparative analysis of nucleotide translocation through protein nanopores using steered molecular dynamics and an adaptive biasing force.Journal of computational chemistry · 2014Article
- DING proteins: numerous functions, elusive genes, a potential for health.Cellular and molecular life sciences : CMLS · 2013Review
- Use of a Pteridine Moiety to Track DNA Uptake in Cells.Pteridines · 2013Article
- Oligonucleotide conjugates for therapeutic applications.Therapeutic delivery · 2013Review
- In vivo SELEX for Identification of Brain-penetrating Aptamers.Molecular therapy. Nucleic acids · 2013Article
- DNA-fragments are transcytosed across CaCo-2 cells by adsorptive endocytosis and vesicular mediated transport.PloS one · 2013Article
- Cellular uptake and intracellular trafficking of antisense and siRNA oligonucleotides.Bioconjugate chemistry · 2012Review
- Applications of biological pores in nanomedicine, sensing, and nanoelectronics.Current opinion in biotechnology · 2010Review
- ATP as a mediator of macula densa cell signalling.Purinergic signalling · 2009Article
- Passage times for polymer translocation pulled through a narrow pore.Biophysical journal · 2008Article
- Glycotargeting to improve cellular delivery efficiency of nucleic acids.Glycoconjugate journal · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have identified a 45-kDa protein purified from rat renal brush border membrane that binds short single-stranded nucleic acid sequences. This activity was purified, reconstituted in proteoliposomes, and then fused with model planar lipid bilayers. In voltage-clamp experiments, the reconstituted 45-kDa protein functioned as a gated channel that allows the passage of nucleic acids. Channel activity was observed immediately after addition of oligonucleotide. Channel activity was not observed in the absence of purified protein or of oligonucleotide or when protein was heat-inactivated prior to forming proteoliposomes. In the presence of symmetrical buffered solution and oligonucleotide, current passed linearly over the range of holding potentials tested. Conductance was 10.4 +/- 0.4 picosiemens (pS) and reversal potential was 0.2 +/- 1.7 mV. There was no difference in channel conductance or reversal potential between phosphodiester and phosphorothioate oligonucleotides. Ion-substitution experiments documented a shift in reversal potential only when a concentration gradient for oligonucleotide was established, indicating that movement of oligonucleotide alone was responsible for current. Movement of oligonucleotide across the bilayer was confirmed by using 32P-labeled oligonucleotides. Channel open probability decreased significantly in the presence of heparan sulfate. These studies provide evidence for a cell surface channel that conducts nucleic acids.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.