ArticleProceedings of the National Academy of Sciences of the United States of America1998
An in vitro blood-brain barrier model: cocultures between endothelial cells and organotypic brain slice cultures.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 63 citations in OpenAlex.
- HumanBioactive materials · 2026Article
- The Blood-Labyrinth Barrier: Non-Invasive Delivery Strategies for Inner Ear Drug Delivery.Pharmaceutics · 2025Review
- The neurovascular unit in leukodystrophies: towards solving the puzzle.Fluids and barriers of the CNS · 2022Review
- Study of BBB Dysregulation in Neuropathogenicity Using Integrative Human Model of Blood-Brain Barrier.Frontiers in cellular neuroscience · 2022Review
- Neuroprotection Mediated by Human Blood Plasma in Mouse Hippocampal Slice Cultures and in Oxidatively Stressed Human Neurons.International journal of molecular sciences · 2021Article
- Brain vascular heterogeneity: implications for disease pathogenesis and design of in vitro blood-brain barrier models.Fluids and barriers of the CNS · 2018Review
- Organotypic brain explant culture as a drug evaluation system for malignant brain tumors.Cancer medicine · 2017Article
- Culture media-based selection of endothelial cells, pericytes, and perivascular-resident macrophage-like melanocytes from the young mouse vestibular system.Hearing research · 2017Article
- Pathophysiology of the cochlear intrastrial fluid-blood barrier (review).Hearing research · 2016Review
- Three-Dimensional Blood-Brain Barrier Model for in vitro Studies of Neurovascular Pathology.Scientific reports · 2015Article
- Organotypic brain slice cultures: A review.Neuroscience · 2015Review
- Organotypic brain slice cultures as a model to study angiogenesis of brain vessels.Frontiers in cell and developmental biology · 2015Article
- Dopamine increases CD14+CD16+ monocyte migration and adhesion in the context of substance abuse and HIV neuropathogenesis.PloS one · 2015Article
- Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear.Nature protocols · 2013Article
- A simple method for measuring organotypic tissue slice culture thickness.Journal of neuroscience methods · 2011Article
- Slice cultures as a model to study neurovascular coupling and blood brain barrier in vitro.Cardiovascular psychiatry and neurology · 2011Article
- TAT-mediated intracellular protein delivery to primary brain cells is dependent on glycosaminoglycan expression.Biotechnology and bioengineering · 2009Article
- Neuron-glial interactions in blood-brain barrier formation.Annual review of neuroscience · 2007Review
- Permeability studies on in vitro blood-brain barrier models: physiology, pathology, and pharmacology.Cellular and molecular neurobiology · 2005Review
- Dynamics of CNS barriers: evolution, differentiation, and modulation.Cellular and molecular neurobiology · 2005Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This communication describes a novel in vitro blood-brain barrier (BBB) model: organotypic slice cultures from the central nervous system were overlaid on endothelial cell monolayers grown on permeable membranes. Morphological, electrophysiological, and microdialysis approaches were carried out to characterize and validate this model. After 10 days in coculture, morphological studies reveal the presence of tight junctions. Electrophysiological recordings of neuronal activity performed on organotypic cultures with or without an endothelial cell monolayer show that amplitude of evoked responses were comparable, indicating good viability of cocultures after 2 weeks. Perfusion of known BBB permeable or nonpermeable molecules was used to test the coculture tightness in conjunction with electrophysiological or microdialysis approaches: application of glutamate (Glu), which doesn't easily cross the BBB, triggers off rhythmic activity only in control cultures, whereas epileptogenic activity was observed in both control cultures and cocultures during perfusions with picrotoxin, a molecule that can diffuse through the BBB. Finally, the microdialysis technique was used to determine the permeability of molecules coming from the perfusion chamber: L-dopa, dopamine, and Glu were employed to assess the selective permeability of the coculture model. Thus, these results indicate that the in vitro model described possesses characteristics similar to those of the BBB in situ and that cocultures of organotypic slices and endothelial cell monolayers have potential as a powerful tool for studying biochemical mechanisms regulating BBB function and drug delivery to the central nervous system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.