ArticleProceedings of the National Academy of Sciences of the United States of America1998
Conformation sensitive gel electrophoresis for simple and accurate detection of mutations: comparison with denaturing gradient gel electrophoresis and nucleotide sequencing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 236 citations in OpenAlex.
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- A novel MTTT mutation m.15933G > A revealed in analysis of mitochondrial DNA in patients with suspected mitochondrial disease.BMC medical genetics · 2017Article
- Association of PALB2 sequence variants with the risk of familial and early-onset breast cancer in a South-American population.BMC cancer · 2015Article
- Mitochondrial DNA variant m.15218A > G in Finnish epilepsy patients who have maternal relatives with epilepsy, sensorineural hearing impairment or diabetes mellitus.BMC medical genetics · 2013Article
- Prevalence of germline TP53 mutations in HER2+ breast cancer patients.Breast cancer research and treatment · 2013Article
- A novel mutation of myelin protein zero associated with late-onset predominantly axonal Charcot-Marie-Tooth disease.Journal of neurology · 2012Article
- Mitochondrial DNA sequence variation in Finnish patients with matrilineal diabetes mellitus.BMC research notes · 2012Article
- Experimental evolution of Legionella pneumophila in mouse macrophages leads to strains with altered determinants of environmental survival.PLoS pathogens · 2012Article
- Breast cancer risk for noncarriers of family-specific BRCA1 and BRCA2 mutations: findings from the Breast Cancer Family Registry.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2011Article
- Mutation screening of the RNF8, UBC13 and MMS2 genes in Northern Finnish breast cancer families.BMC medical genetics · 2011Article
- Variable bone fragility associated with an Amish COL1A2 variant and a knock-in mouse model.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2010Article
- Mutation analysis of the AATF gene in breast cancer families.BMC cancer · 2009Article
- A novel microRNA targeting HDAC5 regulates osteoblast differentiation in mice and contributes to primary osteoporosis in humans.The Journal of clinical investigation · 2009Article
- BRCA1 and BRCA2 mutation carriers in the Breast Cancer Family Registry: an open resource for collaborative research.Breast cancer research and treatment · 2009Article
- Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function.Oncogene · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Previously, an assay called conformation sensitive gel electrophoresis (CSGE) was developed for scanning PCR products for the presence of single-base and larger base mismatches in DNA. The assay was based on the assumption that mildly denaturing solvents in an appropriate buffer can accentuate the conformational changes produced by single-base mismatches in double-stranded DNA and thereby increase the differential migration in electrophoretic gels of heteroduplexes and homoduplexes. Here the sensitivity of assays by CSGE was improved by limiting the maximal size of the PCR products to 450 bp and making several changes in the conditions for PAGE. With the improved conditions, CSGE detected all 76 previously identified single-base changes in a large series of PCR products from collagen genes that contain multiple exons with highly repetitive and GC-rich sequences. In a survey of 736 alleles of collagen genes, CSGE detected 223 unique single-base mismatches that were confirmed by nucleotide sequencing. CSGE has the advantage over other methods for scanning PCR products in that it is simple, requires no special preparation of PCR products, has a large capacity, and does not use radioactivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.