ArticleProceedings of the National Academy of Sciences of the United States of America1998
Energy-transducing thylakoid membranes remain highly impermeable to ions during protein translocation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- New insights into the Tat protein transport cycle from characterizing the assembled Tat translocon.Molecular microbiology · 2022Article
- Oligomerization state of the functional bacterial twin-arginine translocation (Tat) receptor complex.Communications biology · 2022Article
- Electrochromic shift supports the membrane destabilization model of Tat-mediated transport and shows ion leakage during Sec transport.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Evaluating the Functional Pore Size of Chloroplast TOC and TIC Protein Translocons: Import of Folded Proteins.The Plant cell · 2018Article
- Clustering of C-terminal stromal domains of Tha4 homo-oligomers during translocation by the Tat protein transport system.Molecular biology of the cell · 2009Article
- The chloroplast Tat pathway utilizes the transmembrane electric potential as an energy source.Biophysical journal · 2007Article
- Evidence for a dynamic and transient pathway through the TAT protein transport machinery.The EMBO journal · 2007Article
- Thylakoid targeting of Tat passenger proteins shows no delta pH dependence in vivo.The EMBO journal · 2003Article
- A twin arginine signal peptide and the pH gradient trigger reversible assembly of the thylakoid [Delta]pH/Tat translocase.The Journal of cell biology · 2002Article
- Acyl-CoA oxidase is imported as a heteropentameric, cofactor-containing complex into peroxisomes of Yarrowia lipolytica.The Journal of cell biology · 2002Article
- Functional reconstitution of bacterial Tat translocation in vitro.The EMBO journal · 2001Article
- The maize tha4 gene functions in sec-independent protein transport in chloroplasts and is related to hcf106, tatA, and tatB.The Journal of cell biology · 1999Article
- Component specificity for the thylakoidal Sec and Delta pH-dependent protein transport pathways.The Journal of cell biology · 1999Article
- Protein import and routing systems of chloroplasts.The Plant cell · 1999Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the operation of a posttranslational protein translocation pathway to determine whether ions are excluded from the translocase during protein transport. The membrane capacitance during protein translocation across chloroplast thylakoid membranes was monitored via electric-field-indicating carotenoid electrochromic bandshift measurements. Evidence is presented that shows that the membrane ion conductance is not increased during the complete cycle of binding, transport, and substrate release by the DeltapH-dependent translocase; i.e., the membrane remains ion-tight during protein translocation. We further demonstrate that a synthetic targeting peptide that directs proteins across this membrane does not gate translocation pores. We conclude that protein transport across the thylakoid membrane does not compromise its ability to maintain ion gradients and is, thus, unlikely to affect its functions in energy transduction.
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