ArticleProceedings of the National Academy of Sciences of the United States of America1998
Signaling pathways underlying eosinophil cell motility revealed by using caged peptides.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 73 citations in OpenAlex.
- Article
- Caged compounds for multichromic optical interrogation of neural systems.The European journal of neuroscience · 2015Article
- Membrane-tethered ligands: tools for cell-autonomous pharmacological manipulation of biological circuits.Physiology (Bethesda, Md.) · 2013Review
- Structural and molecular micropatterning of dual hydrogel constructs for neural growth models using photochemical strategies.Biomedical microdevices · 2013Article
- Chemical tools for studying directed cell migration.ACS chemical biology · 2011Review
- Light-mediated remote control of signaling pathways.Biochimica et biophysica acta · 2010Review
- Photo-targeted nanoparticles.Nano letters · 2010Article
- Multiple proteins mediate IQGAP1-stimulated cell migration.Cellular signalling · 2007Article
- Caged compounds: photorelease technology for control of cellular chemistry and physiology.Nature methods · 2007Article
- Photochemically-activated probes of protein-protein interactions.Organic letters · 2007Article
- Calcium at fertilization and in early development.Physiological reviews · 2006Review
- Light-activated hydrogel formation via the triggered folding and self-assembly of a designed peptide.Journal of the American Chemical Society · 2005Article
- Distinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts.The Journal of cell biology · 2004Article
- Local photorelease of caged thymosin beta4 in locomoting keratocytes causes cell turning.The Journal of cell biology · 2001Article
- Localization and activity of myosin light chain kinase isoforms during the cell cycle.The Journal of cell biology · 2000Article
- A tribute to Fredric Stewart Fay: June 5, 1943 - March 18, 1997.Biophysical journal · 1998Article
- Myosin light chain kinases: division of work in cell migration.Cell adhesion & migrationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insights into structure-function relations of many proteins opens the possibility of engineering peptides to selectively interfere with a protein's activity. To facilitate the use of peptides as probes of cellular processes, we have developed caged peptides whose influence on specific proteins can be suddenly and uniformly changed by near-UV light. Two peptides are described which, on photolysis of a caging moiety, block the action of calcium-calmodulin or myosin light chain kinase (MLCK). The efficacy of theses peptides is demonstrated in vitro and in vivo by determining their effect before and after photolysis on activities of isolated enzymes and cellular functions known to depend on calcium-calmodulin and MLCK. These caged peptides each were injected into motile, polarized eosinophils, and when exposed to light promptly blocked cell locomotion in a similar manner. The results indicate that the action of calcium-calmodulin and MLCK, and by inference myosin II, are required for the ameboid locomotion of these cells. This methodology provides a powerful means for assessing the role of these and other proteins in a wide range of spatio-temporally complex functions in intact living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.