ArticleProceedings of the National Academy of Sciences of the United States of America1998
DNA-binding domains of Fos and Jun do not induce DNA curvature: an investigation with solution and gel methods.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Control of Recombination Directionality by the Listeria Phage A118 Protein Gp44 and the Coiled-Coil Motif of Its Serine Integrase.Journal of bacteriology · 2017Article
- DNA Sequence Determinants Controlling Affinity, Stability and Shape of DNA Complexes Bound by the Nucleoid Protein Fis.PloS one · 2016Article
- NMR analysis of duplex d(CGCGATCGCG)2 modified by Lambda- and Delta-[Ru(bpy)2(m-GHK)]Cl2 and DNA photocleavage study.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2007Article
- DNA bending by bHLH charge variants.Nucleic acids research · 2006Article
- Asymmetric recognition of nonconsensus AP-1 sites by Fos-Jun and Jun-Jun influences transcriptional cooperativity with NFAT1.Molecular and cellular biology · 2003Article
- Magnesium is required for specific DNA binding of the CREB B-ZIP domain.Nucleic acids research · 2002Article
- Experimental evaluation of the Liu-Beveridge dinucleotide step model of DNA structure.Nucleic acids research · 2001Article
- Global structure and mechanical properties of a 10-bp nucleosome positioning motif.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Design and calibration of a semi-synthetic DNA phasing assay.Nucleic acids research · 2000Article
- Bending and adaptability to proteins of the cAMP DNA-responsive element: molecular dynamics contrasted with NMR.Biophysical journal · 2000Article
- Transcriptional activation by LR1 at the Emu enhancer and switch region sites.Nucleic acids research · 2000Article
- Similarities and differences in the conformation of protein-DNA complexes at the U1 and U6 snRNA gene promoters.Nucleic acids research · 2000Article
- Interaction of calicheamicin gamma1(I) and its related carbohydrates with DNA-protein complexes.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- The SKN-1 amino-terminal arm is a DNA specificity segment.Molecular and cellular biology · 1999Article
- Targeting of the visna virus tat protein to AP-1 sites: interactions with the bZIP domains of fos and jun in vitro and in vivo.Journal of virology · 1999Article
- DNA curvature and deformation in protein-DNA complexes: a step in the right direction.Proceedings of the National Academy of Sciences of the United States of America · 1998Review
- The orientation of the AP-1 heterodimer on DNA strongly affects transcriptional potency.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Molecular basis of cooperative DNA bending and oriented heterodimer binding in the NFAT1-Fos-Jun-ARRE2 complex.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Characterization of the ATF/CREB site and its complex with GCN4.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We demonstrate the use of a DNA minicircle competition binding assay, together with DNA cyclization kinetics and gel-phasing methods, to show that the DNA-binding domains (dbd) of the heterodimeric leucine zipper protein Fos-Jun do not bend the AP-1 target site. Our DNA constructs contain an AP-1 site phased by 1-4 helical turns against an A-tract-directed bend. Competition binding experiments reveal that (dbd)Fos-Jun has a slight preference for binding to linear over circular AP-1 DNAs, independent of whether the site faces in or out on the circle. This result suggests that (dbd)Fos-Jun slightly stiffens rather than bends its DNA target site. A single A-tract bend replacing the AP-1 site is readily detected by its effect on cyclization kinetics, in contrast to the observations for Fos-Jun bound at the AP-1 locus. In contrast, comparative electrophoresis reveals that Fos-Jun-DNA complexes, in which the A-tract bend is positioned close (1-2 helical turns) to the AP-1 site, show phase-dependent variations in gel mobilities that are comparable with those observed when a single A-tract bend replaces the AP-1 site. Whereas gel mobility variations of Fos-Jun-DNA complexes decrease linearly with increasing Mg2+ contained in the gel, the solution binding preference of (dbd)Fos-Jun for linear over circular DNAs is independent of Mg2+ concentration. Hence, gel mobility variations of Fos-Jun-DNA complexes are not indicative of (dbd)Fos-Jun-induced DNA bending (upper limit 5 degrees) in the low salt conditions of gel electrophoresis. Instead, we propose that the gel anomalies depend on the steric relationship of the leucine zipper region with respect to a DNA bend.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.