ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Ca2+ signals mediated by P2X-type purinoceptors in cultured cerebellar Purkinje cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- Drosophila taste neurons as an agonist-screening platform for P2X receptors.Scientific reports · 2020Article
- Specific Temporal Distribution and Subcellular Localization of a Functional Vesicular Nucleotide Transporter (VNUT) in Cerebellar Granule Neurons.Frontiers in pharmacology · 2017Article
- P2Y Receptors in Synaptic Transmission and Plasticity: Therapeutic Potential in Cognitive Dysfunction.Neural plasticity · 2016Review
- Neurochemical and electrical modulation of the locus coeruleus: contribution to CO2drive to breathe.Frontiers in physiology · 2014Review
- Purine receptor-mediated endocannabinoid production and retrograde synaptic signalling in the cerebellar cortex.British journal of pharmacology · 2011Article
- Mouse Leydig cells express multiple P2X receptor subunits.Purinergic signalling · 2009Article
- Dinucleoside polyphosphates and their interaction with other nucleotide signaling pathways.Pflugers Archiv : European journal of physiology · 2006Review
- Modulation of synaptic activity in Purkinje neurons by ATP.Cerebellum (London, England) · 2006Review
- Downstream regulatory element antagonist modulator regulates Ca2+ homeostasis and viability in cerebellar neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Purinergic modulation of cardiovascular function in the rat locus coeruleus.British journal of pharmacology · 2005Article
- Cerebellar astrocytes co-express several ADP receptors. Presence of functional P2Y(13)-like receptors.Purinergic signalling · 2005Article
- Metabotropic P2Y purinoceptor-mediated presynaptic and postsynaptic enhancement of cerebellar GABAergic transmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Role of calcium, glutamate neurotransmission, and nitric oxide in spreading acidification and depression in the cerebellar cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Distinct Localization of P2X receptors at excitatory postsynaptic specializations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Single channel properties of P2X ATP receptors in outside-out patches from rat hippocampal granule cells.The Journal of physiology · 2000Article
- In search of selective P2 receptor ligands: interaction of dihydropyridine derivatives at recombinant rat P2X(2) receptors.Journal of the autonomic nervous system · 2000Article
- ATP-mediated glia signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Functional characterization of the P2X(4) receptor orthologues.British journal of pharmacology · 2000Article
- Ca2+-permeable P2X receptor channels in cultured rat retinal ganglion cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Evidence that multiple P2X purinoceptors are functionally expressed in rat supraoptic neurones.The Journal of physiology · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied [Ca2+]i signals elicited by extracellular ATP in cultured cells from postnatal day 7-8 rat cerebellum using single-cell fluorescence microscopy and fura-2. Putative Purkinje cells selected under phase contrast by size and characteristic cytoplasm appearance were uniquely identified by selective labeling with anti-calbindin antibodies. Extracellularly applied ATP (50 microM) evoked fast [Ca2+]i rises revealed by a rapid and transient increase in fura-2 F340/F380 ratio in all Purkinje cells tested, whereas granule cells failed to show a response to ATP. The mean [Ca2+]i increase was approximately 400 nM, comparable to that obtained after glutamate stimulation. The response to ATP was completely abolished by removal of extracellular Ca2+ with EGTA. Conversely, an increased extracellular Mn2+ entry pathway was activated by ATP stimulation. These results indicate that the effect of ATP is mediated by an ionotropic P2X receptor. The action of ATP was mimicked by the analog 2-methylthio-adenosine 5'-triphosphate with similar efficacy but almost half its potency (EC50, 10.6 +/- 0.7 vs 21.7 +/- 1.9 microM). Other purinergic compounds tested, such as adenosine(5')-tetraphospho-(5')adenosine, adenosine(5')pentaphospho-(5')adenosine, adenosine 5'-(alpha, beta-methylene) triphosphate, UTP, and adenosine, were completely inactive in eliciting [Ca2+]i responses. The purinoceptor antagonists suramin and pyridoxalphosphate-6-azophenyl-2', 4'disulphonic acid effectively blocked the responses elicited by ATP. Our results demonstrate for the first time the presence of functional ionotropic P2X purinoceptors in the cerebellar Purkinje cells and indicate that their pharmacology is similar to receptors formed by P2X2 subunit oligomers.
Indexed as
Identifiers
9464995PMC6792619W2122832621What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.