ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Cytoskeletal actin gates a Cl- channel in neocortical astrocytes.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 76 citations in OpenAlex.
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- Isoflurane impairs immature astroglia development in vitro: the role of actin cytoskeleton.Journal of neuropathology and experimental neurology · 2011Article
- Botulinum neurotoxin devoid of receptor binding domain translocates active protease.PLoS pathogens · 2008Article
- Effect of P2 receptor on the intracellular calcium increase by cancer cells in human umbilical vein endothelial cells.Naunyn-Schmiedeberg's archives of pharmacology · 2008Article
- Thyroid hormones reorganize the cytoskeleton of glial cells through Gfap phosphorylation and Rhoa-dependent mechanisms.Cellular and molecular neurobiology · 2007Article
- Cytoskeleton as a potential target in the neuropathology of maple syrup urine disease: insight from animal studies.Journal of inherited metabolic disease · 2007Review
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- Characterization of Clostridial botulinum neurotoxin channels in neuroblastoma cells.Neurotoxicity research · 2006Article
- Propionic acid induces cytoskeletal alterations in cultured astrocytes from rat cerebral cortex.Metabolic brain disease · 2006Article
- Morphological alterations and cell death provoked by the branched-chain alpha-amino acids accumulating in maple syrup urine disease in astrocytes from rat cerebral cortex.Cellular and molecular neurobiology · 2005Article
- Article
- Expression of voltage-gated chloride channels in human glioma cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Volume-activated chloride currents contribute to the resting conductance and invasive migration of human glioma cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Glutamate release through volume-activated channels during spreading depression.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Increases in astroglial Cl- conductance accompany changes in cell morphology and disassembly of cytoskeletal actin, but Cl- channels underlying these conductance increases have not been described. We characterize an outwardly rectifying Cl- channel in rodent neocortical cultured astrocytes and describe how cell shape and cytoskeletal actin modulate channel gating. In inside-out patch-clamp recordings from cultured astrocytes, outwardly rectifying Cl- channels either were spontaneously active or inducible in quiescent patches by depolarizing voltage steps. Average single-channel conductance was 36 pS between -60 and -80 mV and was 75 pS between 60 and 80 mV in symmetrical (150 mM NaCl) solutions. The permeability ratio (PNa/PCl) was 0.14 at lower ionic strength but increased at higher salt concentrations. Both ATP and 4, 4-diisothiocyanostilbene-2,2'-disulfonic acid produced a flicker block, whereas Zn2+ produced complete inhibition of channel activity. The frequency of observing both spontaneous and inducible Cl- channel activity was markedly higher in stellate than in flat, polygonally shaped astrocytes. In addition, cytoskeletal actin modulated channel open-state probability (PO) and conductance at negative membrane potentials, controlling the degree of outward rectification. Direct application of phalloidin, which stabilizes actin, preserved low PO and promoted lower conductance levels at negative potentials. Lower PO also was induced by direct application of polymerized actin. The actions of phalloidin and actin were reversed by coapplication of gelsolin and cytochalasin D, respectively. These results provide the first report of an outwardly rectifying Cl- channel in neocortical astrocytes and demonstrate how changes in cell shape and cytoskeletal actin may control Cl- conductance in these cells.
Indexed as
Identifiers
9464993PMC2712127W2166682477What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.