ArticleGut1997
Cytokine mRNA expression in intestinal tissue of interleukin-2 deficient mice with bowel inflammation.
Article in Gut, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 69 citations in OpenAlex.
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- Implications the Role of miR-155 in the Pathogenesis of Autoimmune Diseases.Frontiers in immunology · 2021Review
- Taenia solium and Taenia crassiceps: miRNomes of the larvae and effects of miR-10-5p and let-7-5p on murine peritoneal macrophages.Bioscience reports · 2019Article
- An endogenous aryl hydrocarbon receptor ligand, ITE, induces regulatory T cells and ameliorates experimental colitis.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
- Genistein induces macrophage polarization and systemic cytokine to ameliorate experimental colitis.PloS one · 2018Article
- Differential humoral and cellular immunity induced by vaccination using plasmid DNA and protein recombinant expressing the NS3 protein of dengue virus type 3.Journal of biomedical science · 2016Article
- miR-155 deficiency protects mice from experimental colitis by reducing T helper type 1/type 17 responses.Immunology · 2014Article
- Leptin antagonist ameliorates chronic colitis in IL-10⁻/⁻ mice.Immunobiology · 2013Article
- Intestinal infection with Trichinella spiralis induces distinct, regional immune responses.Veterinary parasitology · 2013Article
- Effect of route of infection on outcome of Toxoplasma gondii infection in hu-PBL SCID mice.European journal of microbiology & immunology · 2013Article
- Tregs are dysfunctional in vivo in a spontaneous murine model of Crohn's disease.Mucosal immunology · 2013Article
- Intestinal colonization of IL-2 deficient mice with non-colitogenic B. vulgatus prevents DC maturation and T-cell polarization.PloS one · 2008Article
- The ICE inhibitor pralnacasan prevents DSS-induced colitis in C57BL/6 mice and suppresses IP-10 mRNA but not TNF-alpha mRNA expression.Digestive diseases and sciences · 2007Article
- Interferon gamma downregulates IL-8 production in primary human colonic epithelial cells without induction of apoptosis.International journal of colorectal disease · 2004Article
- An abundance of Escherichia coli is harbored by the mucosa-associated bacterial flora of interleukin-2-deficient mice.Infection and immunity · 2004Article
- In vivo mucosal delivery of bioactive human interleukin 1 receptor antagonist produced by Streptococcus gordonii.BMC biotechnology · 2003Article
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- Nonlymphocyte-derived tumor necrosis factor is required for induction of colitis in recombination activating gene (RAG)2(-/-) mice upon transfer of CD4(+)CD45RB(hi) T cells.The Journal of experimental medicine · 1999Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMice deficient in interleukin-2 (IL-2) develop inflammatory bowel disease resembling ulcerative colitis in humans. Recent studies provided evidence that alpha beta T cells, particularly CD4 T cells, rather than B cells, are involved in the pathogenesis of bowel inflammation of IL-2 deficient mice.
aimTo analyse the pattern of expression of cytokine mRNA in intestinal tissue of normal and IL-2 deficient mice.
methodsExpression of beta-actin, IL-1 alpha, IL-1 beta, IL-6, IL-10, tumour necrosis factor alpha (TNF-alpha), interferon gamma (IFN-gamma) and transforming growth factor beta 1 (TGF-beta 1) mRNA was analysed in colon and small intestinal tissue of both IL-2 deficient (IL-2-/-) mice and normal (wild type) litter mates (IL-2+/+) at different ages by using qualitative, as well as semiquantitative, competitive reverse transcription polymerase chain reaction (RT-PCR). Results were correlated with the phase of progression of the disease, as determined by histology.
resultsIL-2-/- mice had expressed low levels of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, and IFN-gamma mRNA in the colon by 1.5 weeks of age. In advance of the development of histologically and clinically detectable bowel inflammation, expression of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, IFN-gamma, and IL-10, but not TGF-beta 1, mRNA increased in the colon of IL-2 deficient mice. In contrast, IL-2+/+ mice expressed TGF-beta 1 mRNA in colon tissue at 13 and 23 weeks of age, but not IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, IL-10, or IFN-gamma mRNA. Levels of expression of cytokine mRNA in tissue from the small intestine were comparable in IL-2-/- and IL-2+/+ mice.
conclusionsBowel inflammation in IL-2 deficient mice is preceded by an increase in IL-1 alpha, IL-1 beta, TNF-alpha, and IFN-gamma mRNA expression in colon tissue. Low levels of TGF-beta 1, but high levels of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, IFN-gamma, and IL-10 mRNA expression correlate with the manifestation of severe colitis, and suggest that T cells and macrophages are involved in bowel inflammation of IL-2 deficient mice.
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