ArticleThe Biochemical journal1998
Effects of noradrenaline on the cell-surface glucose transporters in cultured brown adipocytes: novel mechanism for selective activation of GLUT1 glucose transporters.
Article in The Biochemical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 78 citations in OpenAlex.
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- Neuroendocrine drivers of risk and resilience: The influence of metabolism & mitochondria.Frontiers in neuroendocrinology · 2019Review
- Shifts in metabolic fuel use coincide with maximal rates of ventilation and body surface rewarming in an arousing hibernator.American journal of physiology. Regulatory, integrative and comparative physiology · 2019Article
- Toward the Existence of a Sympathetic Neuroplasticity Adaptive Mechanism Influencing the Immune Response. A Hypothetical View-Part II.Frontiers in endocrinology · 2019Article
- Intracellular glycolysis in brown adipose tissue is essential for optogenetically induced nonshivering thermogenesis in mice.Scientific reports · 2018Article
- Regulation of glycolysis in brown adipocytes by HIF-1α.Scientific reports · 2017Article
- Systemic Glucoregulation by Glucose-Sensing Neurons in the Ventromedial Hypothalamic Nucleus (VMH).Journal of the Endocrine Society · 2017Review
- The neuroenergetics of stress hormones in the hippocampus and implications for memory.Frontiers in neuroscience · 2015Review
- Adolescents with clinical type 1 diabetes display reduced red blood cell glucose transporter isoform 1 (GLUT1).Pediatric diabetes · 2014Article
- Brown fat fuel utilization and thermogenesis.Trends in endocrinology and metabolism: TEM · 2014Review
- PDE3 and PDE4 isozyme-selective inhibitors are both required for synergistic activation of brown adipose tissue.Molecular pharmacology · 2013Article
- Targeting presynaptic norepinephrine transporter in brown adipose tissue: a novel imaging approach and potential treatment for diabetes and obesity.Synapse (New York, N.Y.) · 2013Article
- Review analysis of the association between the prevalence of activated brown adipose tissue and outdoor temperature.TheScientificWorldJournal · 2012Article
- Thyroid hormone induced brown adipose tissue and amelioration of diabetes in a patient with extreme insulin resistance.The Journal of clinical endocrinology and metabolism · 2010Article
- Altered energy homeostasis and resistance to diet-induced obesity in KRAP-deficient mice.PloS one · 2009Article
- Optimizing interventions for preventing uptake in the brown adipose tissue in FDG-PET.European journal of nuclear medicine and molecular imaging · 2008Article
- Temporal relation between temperature change and FDG uptake in brown adipose tissue.European journal of nuclear medicine and molecular imaging · 2008Article
- Multiple signalling pathways involved in beta2-adrenoceptor-mediated glucose uptake in rat skeletal muscle cells.British journal of pharmacology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glucose transport into rat brown adipocytes has been shown to be stimulated directly by the sympathetic neurotransmitter, noradrenaline, without a significant increase in the protein content of either GLUT1 or GLUT4 glucose transporter in the plasma membrane [Shimizu, Kielar, Minokoshi and Shimazu (1996) Biochem. J. 314, 485-490]. In the present study, we labelled the exofacial glucose-binding sites of GLUT1 and GLUT4 with a membrane-impermeant photoaffinity reagent, 2-N-[4-(1-azitrifluoroethyl)benzoyl]-[2-3H]1,3-bis- (D-mannos-4-yloxy)-2-propylamine (ATB-[3H]BMPA), to determine which isoform is responsible for the noradrenaline-induced increase in glucose transport into intact brown adipocytes in culture. Insulin stimulated the rate of hexose transport by increasing ATB-[3H]BMPA-labelled cell-surface GLUT4. In contrast, the noradrenaline-induced increase in glucose transport was not accompanied by an increased ATB-[3H]BMPA labelling of GLUT4, nor with an increased amount of GLUT4 in the plasma membrane fraction as assessed by Western blotting, indicating that noradrenaline does not promote the translocation of GLUT4. However, noradrenaline induced an increase in photoaffinity labelling of cell-surface GLUT1 without an apparent increase in the immunoreactive GLUT1 protein in the plasma membrane. This is suggestive of an increased affinity of GLUT1 for the ligand. In fact, the Ki value of non-radioactive ATB-BMPA for 2-deoxy-D-glucose uptake was significantly decreased after treatment of the cells with noradrenaline. The increased photoaffinity labelling of GLUT1 and increased glucose transport caused by noradrenaline were inhibited by a cAMP antagonist, cAMP-S Rp-isomer. These results demonstrate that noradrenaline stimulates glucose transport in brown adipocytes by enhancing the functional activity of GLUT1 through a cAMP-dependent mechanism.
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